FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521429
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG16600.3183559027211758No Hit
CTTATATAGAATCCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13080.25084910889114337No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAA12890.24720527626963593No Hit
CATTTAAACCCTAACAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT10960.210191608061692No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10890.20884914341166294No Hit
GTGTAAAAGGGGCAACAAAGCCCAAAAGACCCACAATTCTTTGACATACT10410.1996436715257494No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT10120.19408203226134332No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG9560.1833423150611109No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9400.18027382443247308No Hit
CTTTTACACAATGTGGATATCCTGCCTTGATGCCTTTATATGCATGTATA9290.17816423712528454No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9290.17816423712528454No Hit
CTCTTATATAGAATCCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9040.17336972051803795No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8740.167616300589342No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8010.15361631209618185No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG7900.15150672478899332No Hit
GTGTCAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC7840.15035604080325413No Hit
ATCTCAATGTTAGTATACCTTGGACTCATAAGGTGGGAAACTTTACTGGG7630.14632864685316696No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7440.14268481423165955No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7140.13693139430296358No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7100.13616427164580414No Hit
GAAAAGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGTATA6880.1319450970314271No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6840.13117797437426762No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA6800.13041085171710817No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC6700.12849304507420953No Hit
CATATAAAGGCATCAAGGCAGGATATCCACATTGTGTAAAAGGGGCAACA6650.1275341417527602No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6310.12101359916690478No Hit
TATCCACATTGTGTAAAAGGGGCAACAAAGCCCAAAAGACCCACAATTCT6160.11813688920255681No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6090.11679442455252775No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT6010.11526017923820883No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT5980.11468483724533926No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCA5590.10720539133803453No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5590.10720539133803453No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGA5550.10643826868087505No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5520.10586292668800547No Hit
GTTAGTATACCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5310.10183553273791829No Hit
GCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAGCACTCAGGCA5240.10049306808788923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)