FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536844
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33020.615076260515159No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG20430.38055748038536336No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT16060.2991558069010737No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG14620.27233237216025513No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT14590.2717735506031547No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTGTATGCATGTAT12910.24047954340553307No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12560.23395995857269525No Hit
ATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT12290.22893056455879174No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG10820.20154830826087283No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT10210.19018560326649828No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG10200.18999932941413147No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG10100.18813659089046353No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9590.17863662441975695No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9530.17751898130555618No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG9270.17267586114401948No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9240.17211703958691912No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT9140.17025430106325115No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT8250.15367592820260634No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAACTTCCTATAA8070.15032299886000403No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC7920.14752889107450207No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT7800.14529360484610054No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7510.13989166312746346No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT7480.1393328415703631No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC7410.1380289246037955No Hit
ATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTCTTTGGGT7370.13728382919432833No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA7210.1343034475564596No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA7020.1307642443614905No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGTCAACTTACAAGGCC6880.12815641042835535No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6790.12647994575705418No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTT6710.12498975493811984No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA6690.12461720723338625No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6630.12349956411918546No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6520.12145055174315073No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA6490.12089173018605033No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA6450.12014663477658313No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT6430.11977408707184954No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGACGA6410.11940153936711595No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC6370.11865644395764878No Hit
GGTCTATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAAT6180.11511724076267967No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA6020.11213685912481093No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT6020.11213685912481093No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC5970.11120548986297694No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5880.10952902519167579No Hit
CCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCG5850.1089702036345754No Hit
CATTTAAAAGAGGATATTATCAATAGATGTCAACAATATGTGGGCCCTCT5790.10785256052037463No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC5760.10729373896327425No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5560.10356826191593833No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5510.10263689265410435No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5480.10207807109700398No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5450.10151924953990359No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT5440.10133297568753678No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5420.10096042798280319No Hit
GTAGAGTCCCAAGCGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCTG5370.10002905872096922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)