FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382533
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT11980.3131755953081172No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11430.2987977507822855No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAA9830.2569712939798658No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA9670.25278864829962383No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9570.2501744947494726No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9360.24468477229415503No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGA8670.22664711279811153No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG8650.2261242820880813No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8580.22429437460297544No Hit
ATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT8380.219066067502673No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7780.2033811462017656No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTAAGGTT7740.20233548478170513No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7690.2010284080066295No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATGAACTGCATGTTCAGG7640.19972133123155386No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6680.17462545715010208No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTTGTACAAT6490.16965856540481475No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTTGT6420.16782865791970888No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT6170.1612932740443308No Hit
ATTCTATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAAC6070.1586791204941796No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG5960.15580355158901324No Hit
TGATTAACTGCATGTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGG5830.15240515197381663No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5810.1518823212637864No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTTGTACAATA5760.1505752444887108No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTA5550.1450855220333932No Hit
GGTTAGGATAGAATCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5410.14142570706318147No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC5390.1409028763531512No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5170.13515173854281853No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5160.1348903231878034No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5150.1346289078327883No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5070.1325375849926673No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5060.13227616963765218No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTAAGGTTAGGATAGAAT5040.13175333892762195No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5000.13070767750756146No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA4880.12757069324737996No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT4840.12652503182731947No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4630.1210353093720019No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC4600.12025106330695652No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4410.11528417156166919No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA4410.11528417156166919No Hit
GTCTAAGGGCAAATATTTAGTAAGGTTAGGATAGAATCTAGCAGGCATAA4350.11371567943157844No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTAGGGGAGGAGATTA4320.11293143336653308No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAAGAA4310.11267001801151795No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4310.11267001801151795No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTG4240.1108401105264121No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTGAGACAGGAAATGTGAAACCACA4220.11031727981638186No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG4200.1097944491063516No Hit
GGATAGAATCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4180.10927161839632137No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACAAAAA4150.108487372331276No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4120.10770312626623062No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4040.10561180342610964No Hit
ATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTTGTACAATATGTT4030.10535038807109452No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT4010.10482755736106426No Hit
GATTAACTGCATGTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGC3890.1016905731008828No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT3860.10090632703583743No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG3830.10012208097079206No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3830.10012208097079206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)