FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382533
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11290.2951379358120737No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT10620.2776231070260605No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAA9780.25566421720479016No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA9590.25069732545950285No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9560.24991307939445745No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9480.24782175655433647No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATGAACTGCATGTTCAGG8590.22455578995799053No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8330.21775899072759736No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG8310.21723616001756713No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7600.1986756698114934No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGA7510.19632293161635728No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7340.1918788705811002No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTTGTACAATA7180.18769622490085822No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTAAGGTT7130.1863891481257826No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6980.18246791780055577No Hit
ATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT6860.17933093354037427No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6660.17410262644007185No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5880.15371222874889226No Hit
TGATTAACTGCATGTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGG5830.15240515197381663No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTA5720.14952958306865027No Hit
ATTCTATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAAC5660.14796109093855955No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5570.14560835274342346No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTTGTACAAT5560.14534693738840831No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT5480.14325561454828734No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTTGT5070.1325375849926673No Hit
GGTTAGGATAGAATCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4980.1301848467975312No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT4950.12940060073248583No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTAAGGTTAGGATAGAAT4910.12835493931242534No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA4870.12730927789236485No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4770.12469512434221362No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4550.1189439865318809No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTAGGGGAGGAGATTA4540.11868257117686577No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4530.11842115582185067No Hit
GTCTAAGGGCAAATATTTAGTAAGGTTAGGATAGAATCTAGCAGGCATAA4530.11842115582185067No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4470.11685266369175992No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC4440.11606841762671456No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4410.11528417156166919No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC4390.11476134085163893No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTCAAGAA4310.11267001801151795No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4290.11214718730148772No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACAAAAA4290.11214718730148772No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA4250.11110152588142723No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT4190.1095330337513365No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG4180.10927161839632137No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4180.10927161839632137No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTGAGACAGGAAATGTGAAACCACA4150.108487372331276No Hit
GGATAGAATCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4080.10665746484617013No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA4020.10508897271607939No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3950.10325906523097354No Hit
ATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTTGTACAATATGTT3920.10247481916592818No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC3900.10195198845589792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)