FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507720
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38510.758488930906799No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT32790.6458284093594895No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT30310.5969825888284881No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT28330.5579847159851886No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG22410.4413850153627984No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA21810.4295674781375562No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC21530.4240526274324431No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT21100.4155833924210195No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC19800.3899787284329945No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA18470.36378318758370753No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA17710.34881430709840067No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTCGGCGA16520.3253761916016702No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTCGGCGAGAAAGTAA15840.31198298274639563No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAA15340.30213503505869377No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT15170.2987867328448751No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT14000.27574253525565273No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG13750.2708185614118018No Hit
AGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA13720.27022768455053964No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG13690.26963680768927756No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAA13460.2651067517529347No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC13360.2631371622153943No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT13340.26274324430788626No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC12920.25447096825021664No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG12080.2379264161348775No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG12020.23674466241235329No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC12000.2363507445048452No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT11980.23595682659733713No Hit
GTAATTGGGAGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAAAT11800.23241156542976446No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG11280.22216969983455448No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTG11120.2190183565744899No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG11110.21882139762073585No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC11040.21744268494445756No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA10980.21626093122193335No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT10820.21310958796186874No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTCGGCGAGAAAGTA10580.20838257307177185No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC10550.20779169621050975No Hit
AGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA10510.20700386039549357No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA10430.2054281887654613No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT10080.19853462538406996No Hit
GTACAATATGTTCTTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC10060.19814070747656187No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9770.1924288978176948No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG9670.19045930828015442No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC9550.18809580083510596No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAAC9510.1873079650200898No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT9450.18612621129756557No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCGACTTACAAGGCC9370.1845505396675333No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA9300.183171826991255No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC9220.18159615536122273No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC9150.18021744268494447No Hit
CTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTG8920.1756873867486016No Hit
GGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAA8840.17411171511856927No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG8630.1699755770897345No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA8610.16958165918222642No Hit
GTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAA8590.16918774127471836No Hit
GGATATGTAATTGGGAGTTGGGGCACATTGCCACAAGAACATATTGTACA8520.16780902859844007No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT8290.1632789726620972No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG8230.162097218939573No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG8160.16071850626329473No Hit
GGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTA7890.1554006145119357No Hit
CAATATGTTCTTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATG7620.15008272276057671No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7550.14870401008429843No Hit
GTTCAGGAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCA7540.1485070511305444No Hit
CTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCGACT7530.14831009217679036No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT7430.14634050263924997No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG7430.14634050263924997No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG7410.1459465847317419No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTCGGCGAGAAAGTAAAAGCCTGTT7360.1449617899629717No Hit
TTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGA7340.14456787205546365No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG7170.14121956984164502No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC7090.1396438982116127No Hit
TACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAG7090.1396438982116127No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA7090.1396438982116127No Hit
CATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGG7050.13885606239659656No Hit
GTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAAC6860.13511384227526982No Hit
CAAGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGT6700.13196249901520524No Hit
CCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCGAC6700.13196249901520524No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC6690.1317655400614512No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA6550.12900811470889467No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT6490.12782636098637043No Hit
CATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAA6460.12723548412510832No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6460.12723548412510832No Hit
CTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAAGAACATA6390.12585677144883006No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG6350.1250689356338139No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT6330.12467501772630585No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC6320.1244780587725518No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATG6300.12408414086504371No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTC6290.12388718191128968No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6280.12369022295753565No Hit
CCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAA6200.12211455132750335No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCAGGCC6150.12112975655873316No Hit
ATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG6090.11994800283620893No Hit
GGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAAT6080.11975104388245489No Hit
ATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAAACAGG5980.11778145434491452No Hit
ATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCGACTTACA5940.11699361852989837No Hit
ATGTTCTTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAAGT5820.11463011108484991No Hit
GGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAAC5780.11384227526983377No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA5760.1134483573623257No Hit
AACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAAAC5730.11285748050106359No Hit
ATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCATG5650.11128180887103127No Hit
GGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATT5630.1108878909635232No Hit
TTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCATGG5600.11029701410226109No Hit
ATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATACCCAAAGACAAA5580.10990309619475301No Hit
ACAAAAAGATGGGGATATTCCCTTAACTTCATGGGATATGTAATTGGGAG5580.10990309619475301No Hit
CATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACAT5480.10793350665721263No Hit
TTCCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCAC5450.10734262979595054No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA5400.10635783502718034No Hit
CACTTGGCACAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGAT5350.10537304025841014No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5340.10517608130465611No Hit
ATCTTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT5280.1039943275821319No Hit
GAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATACCCAAAGA5240.10320649176711573No Hit
GCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGC5110.10064602536831324No Hit
CTTTCACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA5090.10025210746080516No Hit
GTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA5080.10005514850705112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)