FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519531
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT41030.7897507559702887No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT40430.7782018782324828No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG39650.7631883371733351No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG30670.5903401336975079No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA22390.43096562091578755No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT21400.4119099726484079No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA17640.33953700549149135No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17430.3354948982832593No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA16730.32202120758915254No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC16560.31874902556344087No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT15520.2987309708179108No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA15250.29353397583589813No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14650.2819850980980923No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA14220.27370840238599814No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA13870.26697155703894476No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13770.2650467440826437No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT13420.2583098987355904No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG12540.2413715447201418No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG12160.23405725548619813No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA11900.2290527417998156No Hit
CATTTAAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT11660.22443319070469328No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT11630.22385574681780296No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT11570.2227008590440224No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11450.22039108349646122No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11410.21962115831394083No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC10810.20807228057613503No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT10550.20306776688975248No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC10540.20287528559412238No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT10120.19479107117765831No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10040.19325122081261753No Hit
ACAAAAATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAA9940.19132640785631655No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTACTGGG9920.19094144526505635No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9860.1897865574912758No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC9450.1818948243704418No Hit
GGGTAAAGGTTCAGATATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA9330.17958504882288065No Hit
CCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG9250.17804519845783986No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC9020.1736181286583476No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT8880.17092339051952626No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG8680.16707376460692433No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC8270.15918203148609034No Hit
CCCTTAGACAAAGGCATTAAACCGTATTATCCTGAACATGCAGTTAATCA8210.15802714371230975No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8190.15764218112104955No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8070.1553324055734884No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC8070.1553324055734884No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC8050.1549474429822282No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7870.15148277966088644No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7830.15071285447836605No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7820.15052037318273598No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT7700.1482105976351748No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCAC7680.1478256350439146No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG7660.1474406724526544No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7640.1470557098613942No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT7550.14532337820072336No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7440.14320608394879228No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTTAGT7270.13993390192308064No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7260.13974142062745054No Hit
ATGTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCT7250.13954893933182044No Hit
CTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG7240.13935645803619034No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA7120.13704668248862917No Hit
GATTAACTGCATGTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGC7100.13666171989736897No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG7030.13531435082795829No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTTAGTACAATA6980.13435194434980782No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA6960.1339669817585476No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6950.1337745004629175No Hit
AGTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTTAAATGTATA6930.1333895378716573No Hit
TTCTTCTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTC6840.13165720621098645No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA6780.13050231843720586No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6720.1293474306634253No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6680.12857750548090488No Hit
ACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTT6650.1280000615940146No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGAAAATTGCCTGTAA6630.12761509900275442No Hit
CATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAA6620.12742261770712432No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTTAGTACAAT6500.12511284215956314No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC6420.12357299179452236No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6380.12280306661200198No Hit
GCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAGCACTCAGGCA6320.1216481788382214No Hit
ATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGG6310.12145569754259129No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6290.1210707349513311No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACAG6250.12030080976881072No Hit
ACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA6140.11818351551687964No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6130.11799103422124954No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6130.11799103422124954No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6130.11799103422124954No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCT6090.11722110903872916No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA6040.11625870256057867No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC5830.11221659535234664No Hit
TTCATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCT5770.11106170757856605No Hit
GCATTAAACCGTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACT5710.10990681980478548No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5670.10913689462226508No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT5650.10875193203100487No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT5630.10836696943974469No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT5610.1079820068484845No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5430.10451734352714276No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5410.10413238093588256No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5350.10297749316210197No Hit
ATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTA5280.1016301240926913No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5270.10143764279706119No Hit
ACCTTGAGGCATACTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTG5270.10143764279706119No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG5260.10124516150143109No Hit
GCTAGGTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCAT5220.10047523631891071No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)