FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515503
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTGGAGTTACTCTCTTTTTTACCTTCTGACTTCTTTCCTTCTATTCG37660.7305486098044046No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC32240.625408581521349No Hit
TGCTTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA30060.5831197878576846No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC26320.5105692886365355No Hit
CCTCCAGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA26070.5057196563356566No Hit
TGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA23580.45741731861890234No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT20940.4062052015216206No Hit
CAATCACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG20510.39786383396410885No Hit
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACC19980.38758261348624545No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAA19660.3813750841411204No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG19570.379629216512804No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA18590.3606186578933585No Hit
TCTGGAGAGTTACTCTCTTTTTTACCTTCTGACTTCTTTCCTTCTATTCG18200.3530532315039874No Hit
GAGCAGTAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGAT17260.33481861405268254No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA16460.3192997906898699No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT14210.27565309998195936No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG13940.2704154970970101No Hit
TCCCTTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGA13740.26653579125630694No Hit
ATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAGATCCCAGAGT13510.2620741295394983No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG13110.254314717858092No Hit
CCTCAAGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12970.2515989237695998No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG12190.23646807099085748No Hit
GCCTAGCAGCCATGGAAAGGAGGTATACTTGCGGGAGAGCACGACAGAAT11880.23045452693776758No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC11460.22230714467229096No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT10680.20717629189354864No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA10670.20698230660151348No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC10540.20446049780505643No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA10170.1972830419997556No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA10000.1939852920351579No Hit
CCCACGGGAACCTGCCCAAGGTCTTGCATAAGAGGACTCTTGGACTTTCA9870.19146348323870083No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG9580.1858379097696813No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9570.1856439244776461No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC9400.1823461745130484No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA9390.18215218922101326No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9390.18215218922101326No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG9370.18176421863694295No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA9360.18157023334490777No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG9090.17633263045995853No Hit
ATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGCATCCAGGGAA9040.17536270399978274No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG8960.17381082166350148No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC8860.1718709687431499No Hit
CACTCACCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTG8800.17070705699093894No Hit
GACCAGGCCGTTGCCTAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT8730.16934915994669283No Hit
CTGTTGTACCGTCCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC8680.16837923348651704No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT8630.16740930702634127No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8580.16643938056616547No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA8400.16294764530953265No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC8390.16275366001749747No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG8250.16003786592900526No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8230.15964989534493496No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8190.1588739541767943No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7920.15363635129184505No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT7820.1516964983714935No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7650.1483987484068958No Hit
TCCCGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGA7630.14801077782282546No Hit
TGGTAAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACACGG7560.14665288077857938No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7540.14626491019450905No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG7470.14490701315026294No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG7470.14490701315026294No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA7440.14432505727415748No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7430.14413107198212233No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA7400.14354911610601684No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT7390.14335513081398168No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA7260.14083332201752463No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC7180.13928143968124337No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT7050.13675963088478632No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6960.13501376325646988No Hit
TACACACTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGT6920.13423782208832924No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6830.13249195446001283No Hit
GGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAGATCCCA6730.13055210153966126No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6680.1295821750794855No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6650.12900021920338No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6590.12783630745116906No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC6580.12764232215913388No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6560.1272543515750636No Hit
AAATAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGC6530.1266723956989581No Hit
GAGTATGTAAATAATGTCGTGTCTGGAAGTAATGATTAACTACATACTCT6410.12434457219453621No Hit
CTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCACAACTGTGCCAGC6380.12376261631843073No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6370.12356863102639558No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6280.12182276339807915No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6250.12124080752197368No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTATTTA6240.12104682222993852No Hit
CCACCGCACGGCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAG6230.12085283693790336No Hit
TAGATAAAGGGATCAAACCGTATTATCCAGAGTATGTAGTTAATCATTAC6080.11794305755737601No Hit
ATCTTGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGCATCCAGGGAA6010.11658516051312989No Hit
CCCAAGGGAACCTGCCCAAGGTCTTGCATAAGAGGACTCTTGGACTTTCA5930.11503327817684864No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5930.11503327817684864No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5920.11483929288481347No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5890.114257337008708No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5880.11406335171667284No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5870.11386936642463767No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA5830.11309342525649706No Hit
GAGTATGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCT5800.11251146938039158No Hit
ATGTGATTGGGAGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAA5790.11231748408835641No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC5780.11212349879632126No Hit
GAGTTAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGA5740.11134755762818065No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT5730.11115357233614548No Hit
GGCCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC5720.11095958704411032No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTG5700.11057161646004No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC5660.10979567529189936No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5650.10960168999986421No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5610.10882574883172358No Hit
ACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT5610.10882574883172358No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT5590.10843777824765327No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA5510.10688589591137201No Hit
CCTTCATTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGG5490.10649792532730169No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5490.10649792532730169No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG5480.10630394003526653No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG5470.10610995474323137No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5450.10572198415916105No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC5390.10455807240695011No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5380.10436408711491496No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG5350.10378213123880947No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT5340.10358814594677432No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5340.10358814594677432No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5260.10203626361049306No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA5250.1018422783184579No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT5210.10106633715031726No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5180.10048438127421178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)