FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515503
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC31530.6116356257868528No Hit
TGCTTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA31360.6083378758222552No Hit
TCTGTGGAGTTACTCTCTTTTTTACCTTCTGACTTCTTTCCTTCTATTCG28270.5483964205833913No Hit
CCTCCAGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA26070.5057196563356566No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC25040.4857391712560354No Hit
TGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA24260.470608318477293No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG22290.432393215946367No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAA20820.40387737801719875No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT19910.38622471644199935No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA19000.3685720548668No Hit
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACC17500.33947426106152634No Hit
CAATCACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG16900.32783514353941684No Hit
TCTGGAGAGTTACTCTCTTTTTTACCTTCTGACTTCTTTCCTTCTATTCG14790.28690424691999855No Hit
GAGCAGTAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGAT14440.28011476169876803No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA13910.26983354122090464No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG13580.2634320265837444No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT13520.2622681148315335No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG13380.25955232074304124No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG12770.24771921792889665No Hit
CCTCAAGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12460.24170567387580671No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC11890.23064851222980273No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT11800.2289026446014863No Hit
ATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAGATCCCAGAGT11640.22579887992892378No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11480.2226951152563613No Hit
GCCTAGCAGCCATGGAAAGGAGGTATACTTGCGGGAGAGCACGACAGAAT11400.22114323292008No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT10700.20756426247761894No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC10530.20426651251302125No Hit
TCCCTTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGA10480.20329658605284548No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA9960.19320935086701727No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA9890.19185145382277116No Hit
CTGTTGTACCGTCCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC9590.1860318950617164No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA9550.1852559538935758No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG9260.1796303804245562No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTATTTA9010.17478074812367725No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8950.17361683637146633No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC8940.17342285107943115No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA8830.17128901286704443No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC8720.16915517465465768No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8720.16915517465465768No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG8690.1685732187785522No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8640.16760329231837642No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG8590.16663336585820063No Hit
ATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGCATCCAGGGAA8550.16585742469005998No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8520.16527546881395452No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG8520.16527546881395452No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG8490.16469351293784906No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT8340.16178373355732167No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG8240.1598438806369701No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA8230.15964989534493496No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA8150.1580980130086537No Hit
GACCAGGCCGTTGCCTAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT8120.1575160571325482No Hit
CACTCACCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTG8050.1561581600883021No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7840.1520844689555638No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG7790.151114542495388No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7770.1507265719113177No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7760.1505325866192825No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA7700.1493686748670716No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7430.14413107198212233No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC7430.14413107198212233No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC7260.14083332201752463No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7190.13947542497327853No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT7150.13869948380513789No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA6950.13481977796443473No Hit
ATGTGATTGGGAGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAA6920.13423782208832924No Hit
ATCTTGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGCATCCAGGGAA6770.1313280427078019No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6720.1303581162476261No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6680.1295821750794855No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6650.12900021920338No Hit
GGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAGATCCCA6540.12686638099099326No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6440.1249265280706417No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG6430.12473254277860653No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT6400.12415058690250105No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT6390.1239566016104659No Hit
TACACACTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGT6380.12376261631843073No Hit
GAGTATGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCT6380.12376261631843073No Hit
GAGTTAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGA6340.12298667515029009No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6330.12279268985825496No Hit
GGCCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC6300.12221073398214947No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC6220.1206588516458682No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6220.1206588516458682No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6210.12046486635383306No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6200.1202708810617979No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA6160.11949493989365727No Hit
TGGTAAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACACGG6130.11891298401755178No Hit
CCCACGGGAACCTGCCCAAGGTCTTGCATAAGAGGACTCTTGGACTTTCA6120.11871899872551663No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6110.11852501343348146No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT6100.1183310281414463No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6070.11774907226534084No Hit
CTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCACAACTGTGCCAGC5800.11251146938039158No Hit
CCACCGCACGGCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAG5780.11212349879632126No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC5750.1115415429202158No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5680.11018364587596968No Hit
TCCCGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGA5670.10998966058393453No Hit
CCCCAACACAGAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATG5670.10998966058393453No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5670.10998966058393453No Hit
GAGTATGTAAATAATGTCGTGTCTGGAAGTAATGATTAACTACATACTCT5650.10960168999986421No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5560.10785582237154778No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA5550.10766183707951263No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5530.10727386649544231No Hit
CCCAAGGGAACCTGCCCAAGGTCTTGCATAAGAGGACTCTTGGACTTTCA5510.10688589591137201No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5460.1059159694511962No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA5370.1041701018228798No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC5370.1041701018228798No Hit
CCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCT5370.1041701018228798No Hit
AAATAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGC5350.10378213123880947No Hit
ACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATT5320.103200175362704No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT5300.10281220477863368No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC5290.10261821948659852No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC5290.10261821948659852No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG5280.10242423419456338No Hit
CCTTCATTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGG5260.10203626361049306No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT5220.10126032244235242No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5200.1008723518582821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)