FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517341
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG30980.5988313317521712No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28160.5443218302821543No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCCTAT17280.3340156685822311No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16090.31101343214630195No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA15320.2961296320995243No Hit
CTTATATAGAATTCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15010.2901374528599125No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA14600.2822123125752647No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA13650.26384918264742213No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA12760.24664582934660118No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATACCCACATTGTGTAAAA11830.22866929162776584No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10420.20141454089275737No Hit
CTATTAATAATGTCCTCCTGTAAGTGAATGTGAGGAAAGGAGGGAGTTTG10240.19793521101169248No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT10220.19754861880268526No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9400.18169833823338957No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9400.18169833823338957No Hit
CTCTTATATAGAATTCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9390.181505042128886No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9380.18131174602438238No Hit
ATGTTAATATGGGCCTAAAAATAAGACAACTACTGTGGTTTCACATTTCC9190.17763912003881385No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9170.17725252782980666No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8200.15850280569295688No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC8170.15792291737944605No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8140.15734302906593522No Hit
GGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT8060.1557966602299064No Hit
TACCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT7980.15425029139387755No Hit
ATTTACATACTCTGTGGAAGGCTGGAATTCTATATAAGAGAGAAACTACA7950.15367040308036672No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7890.1525106264533451No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7630.14748492773625133No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7600.14690503942274052No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA7570.1463251511092297No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7540.14574526279571887No Hit
CTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7530.14555196669121526No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7500.14497207837770446No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7500.14497207837770446No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7480.14458548616869724No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7390.14284582122816478No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7380.1426525251236612No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7130.13782012251107104No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC6920.13376090431649532No Hit
CTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTTA6910.1335676082119917No Hit
TCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6910.1335676082119917No Hit
CACTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT6850.13240783158497005No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6760.13066816664443762No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC6750.130474870539934No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6660.12873520559940155No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAAGAACATATTGTACTAAAA6570.1269955406588691No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCACTTACAGG6550.1266089484498619No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGTAGTTTTCGAAAAC6500.12564246792734385No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAGGGTTTAATGCC6260.12100336141925733No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6180.11945699258322846No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6170.11926369647872487No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6150.11887710426971765No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6080.11752403153819241No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5920.11443129386613472No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATAAGACA5910.1142379977616311No Hit
GCATTAAACCCTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACT5870.11346481334361669No Hit
GTTAATATGGGCCTAAAAATAAGACAACTACTGTGGTTTCACATTTCCTG5840.11288492503010586No Hit
GAATATGGTGACCCACAAAATGAAGCACTGCGTGTAGTTTCTCTCTTATA5840.11288492503010586No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC5830.11269162892560228No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5820.11249833282109865No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5810.11230503671659504No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5780.11172514840308424No Hit
GTATAAAGAATTTGGAGCTTCCGTGGAGTTACTCTCTTTTTTGCCTTCTG5770.11153185229858062No Hit
ATGAAGCACTGCGTGTAGTTTCTCTCTTATATAGAATTCCAGCCTTCCAC5730.11075866788056621No Hit
ATTTACAGGTAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATATGTT5690.10998548346255177No Hit
CTTTTACACAATGTGGGTATCCTGCCTTAATGCCTTTATATGCATGTATA5650.10921229904453736No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5580.10785922631301212No Hit
CCTGTAAATAGACCTATTGATTGGAAAGTATGTCAGAGAATTGTGGGTCT5570.10766593020850851No Hit
GTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC5560.10747263410400491No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5550.10727933799950129No Hit
TTTACATACTCTGTGGAAGGCTGGAATTCTATATAAGAGAGAAACTACAC5500.10631285747698327No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC5500.10631285747698327No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAGAGAATTGTGGGTCTTTT5490.10611956137247967No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5420.1047664886409544No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCGCAAGAACATATTGTACT5390.1041866003274436No Hit
ATTTACAGGTAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATATGTT5370.1038000081184364No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5370.1038000081184364No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA5340.10322011980492557No Hit
AAATGAAGCACTGCGTGTAGTTTCTCTCTTATATAGAATTCCAGCCTTCC5300.10244693538691114No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5290.10225363928240755No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG5280.10206034317790393No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5270.10186704707340034No Hit
GCTATGTCAATGTTAATATGGGCCTAAAAATAAGACAACTACTGTGGTTT5250.10148045486439311No Hit
CTATATAAGAGAGAAACTACACGCAGTGCTTCATTTTGTGGGTCACCATA5240.10128715875988951No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC5200.1005139743418751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)