FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517341
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG31890.6164212772619994No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28210.5452883108046724No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCCTAT17010.3287966737606337No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16000.3092737672057695No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA14180.27409387618611325No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA14080.27216091514107715No Hit
CTTATATAGAATTCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13820.2671352164239834No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA13400.259016780034832No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATACCCACATTGTGTAAAA12850.24838549428713363No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA12630.24413297998805433No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT11910.23021566046379466No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10800.2087597928638944No Hit
CTATTAATAATGTCCTCCTGTAAGTGAATGTGAGGAAAGGAGGGAGTTTG10660.20605364740084392No Hit
CTCTTATATAGAATTCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10130.1958089538621528No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9740.1882704057865122No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9630.1861441486369725No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC9070.1753195667847706No Hit
ATGTTAATATGGGCCTAAAAATAAGACAACTACTGTGGTTTCACATTTCC8940.1728067174262237No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8930.1726134213217201No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8900.17203353300820928No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8780.16971397975416602No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8350.16140224726051097No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8120.15695643685692803No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7960.15386369918487033No Hit
CTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7770.15019107319930183No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7750.1498044809902946No Hit
TACCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT7670.14825811215426576No Hit
GGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT7600.14690503942274052No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7490.14477878227320085No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7460.14419889395969002No Hit
ATTTACATACTCTGTGGAAGGCTGGAATTCTATATAAGAGAGAAACTACA7450.14400559785518643No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7240.1399463796606107No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7140.13801341861557465No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA7130.13782012251107104No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC7070.13666034588404938No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCACTTACAGG7050.1362737536750422No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7030.13588716146603497No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7000.13530727315252414No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6950.13434079263000612No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6950.13434079263000612No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6570.1269955406588691No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6550.1266089484498619No Hit
CTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTTA6420.12409609909131501No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC6420.12409609909131501No Hit
CTTTTACACAATGTGGGTATCCTGCCTTAATGCCTTTATATGCATGTATA6420.12409609909131501No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6390.12351621077780418No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6350.12274302635978977No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAGGGTTTAATGCC6320.12216313804627894No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6320.12216313804627894No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC6210.12003688089673929No Hit
GTTAATATGGGCCTAAAAATAAGACAACTACTGTGGTTTCACATTTCCTG6180.11945699258322846No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAAGAACATATTGTACTAAAA6100.11791062374719961No Hit
TCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6090.11771732764269602No Hit
CCTGTAAATAGACCTATTGATTGGAAAGTATGTCAGAGAATTGTGGGTCT5980.11559107049315634No Hit
TTTACATACTCTGTGGAAGGCTGGAATTCTATATAAGAGAGAAACTACAC5830.11269162892560228No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGTAGTTTTCGAAAAC5800.11211174061209145No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT5780.11172514840308424No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5780.11172514840308424No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5780.11172514840308424No Hit
ATTTACAGGTAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATATGTT5770.11153185229858062No Hit
GTCTATTTACAGGTAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA5750.11114526008957341No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG5740.1109519639850698No Hit
CACTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT5740.1109519639850698No Hit
GCATTAAACCCTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACT5720.1105653717760626No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5710.11037207567155898No Hit
GAATATGGTGACCCACAAAATGAAGCACTGCGTGTAGTTTCTCTCTTATA5700.1101787795670554No Hit
GTATAAAGAATTTGGAGCTTCCGTGGAGTTACTCTCTTTTTTGCCTTCTG5650.10921229904453736No Hit
GTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC5630.10882570683553015No Hit
ATTTACAGGTAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATATGTT5620.10863241073102653No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5580.10785922631301212No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5530.10689274579049408No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5510.10650615358148688No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5480.10592626526797605No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5420.1047664886409544No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAGAGAATTGTGGGTCTTTT5400.1043798964319472No Hit
ATGAAGCACTGCGTGTAGTTTCTCTCTTATATAGAATTCCAGCCTTCCAC5370.1038000081184364No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5330.10302682370042196No Hit
GTCTATTTACAGGTAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATA5240.10128715875988951No Hit
GTGTAGTTTCTCTCTTATATAGAATTCCAGCCTTCCACAGAGTATGTAAA5220.10090056655088231No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATAAGACA5210.10070727044637869No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5190.10032067823737148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)