FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454408
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42100.9264801676026831No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA28250.6216879984507315No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC24850.5468653720885196No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA15110.3325205542155948No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC13030.286746712205771No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCCGG12050.26518019048960406No Hit
GTCTTACATAAGCGGACTCTTGGACTCTCAGCGATGTCAACGACCGACCT11190.24625446735092693No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11160.24559426770655446No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC10720.23591133958909177No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10640.2341508072040985No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT10310.22688861111600148No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC10290.22644847801975318No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9280.20422175665921377No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAGGGCATTAAACCTTAT9220.20290135737046885No Hit
CCCTTAACTTTATGGGATATATAATTGGAAGTTGGGGTACTTTACCACAG9040.1989401595042341No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA8610.1894772979348955No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTATATATCCC8590.1890371648386472No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAGGTCTTGCCC8410.18507596697241246No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG8280.18221510184679846No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT8230.18111476910617771No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTATATATCCCATAAA8150.1793542367211845No Hit
GTCCAAGAGTCCGCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC8150.1793542367211845No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA8080.17781377088431544No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC8050.17715357123994296No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT7950.17495290575870143No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7470.1643897114487421No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGTCTTATAAGTTGGCGA7440.16372951180436962No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7320.1610887132268798No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6990.15382651713878276No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6900.1518459182056654No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6900.1518459182056654No Hit
GTACGGGACCATGCAAGACCTGCACGATACCTGCTCAAGGAACCTCTATG6850.15074558546504463No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG6820.15008538582067218No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCT6520.1434833893769476No Hit
GGTCTATTTACAGGAAGTTTTCGAAAGCATTGCTTGATTTTCAGTACAAT6510.14326332282882345No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6500.14304325628069928No Hit
ATATAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACTGAAA6470.14238305663632683No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTGCACTTCGCTTCAC6360.13996232460696115No Hit
TCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAG6280.13820179222196793No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6270.13798172567384379No Hit
CCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAAGAGTCCGCTTATGT6200.1364412598369747No Hit
CAATAGGTCTATTTACAGGAAGTTTTCGAAAGCATTGCTTGATTTTCAGT6100.13424059435573318No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA6020.13248006197073994No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6020.13248006197073994No Hit
ATATTGTACTGAAAATCAAGCAATGCTTTCGAAAACTTCCTGTAAATAGA6010.1322599954226158No Hit
GGAACATATTGTACTGAAAATCAAGCAATGCTTTCGAAAACTTCCTGTAA5990.1318198623263675No Hit
CTCTTGGACTCTCAGCGATGTCAACGACCGACCTTGAGGCATACTTCAAA5870.12917906374887766No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTTCGAAAGC5820.1280787310082569No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5800.1276385979120086No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5780.12719846481576028No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGCGGCCG5710.1256579989788912No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTGTGTTTAAAGACT5620.12367740004577384No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA5620.12367740004577384No Hit
GCTTTTCTGTGTTGGGGTGAGTTGATGAATTTGGCCACCTGGGTGGGAAG5590.12301720040140138No Hit
CTGTAAGAGGGCCTACATAGTTTTGACATCTATTAATAATGTCTTCCTGC5580.12279713385327723No Hit
ACCTTGAGGCATACTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTG5530.12169680111265646No Hit
CAGTTATATGGATGATTTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5500.121036601468284No Hit
GACCTTCAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC5390.11861586943891834No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGAAG5320.11707540360204925No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAGGGCATTAAAC5310.1168553370539251No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA5300.11663527050580097No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5250.11553493776518019No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5230.11509480466893188No Hit
GTGGTAAAGTACCCCAACTTCCAATTATATATCCCATAAAGTTAAGGGAG5180.11399447192831114No Hit
GTCGTTGACATCGCTGAGAGTCCAAGAGTCCGCTTATGTAAGACCTTGGG5150.11333427228393868No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG5090.11201387299519376No Hit
CCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGAAGTATGCCTCA5040.11091354025457298No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT4980.10959314096582806No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4970.10937307441770393No Hit
TTCTACTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4950.1089329413214556No Hit
GTCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTATACATGC4940.10871287477333147No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGACC4930.1084928082252073No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4930.1084928082252073No Hit
ATTTACAGGAAGTTTTCGAAAGCATTGCTTGATTTTCAGTACAATATGTT4910.10805267512895901No Hit
ATTTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4870.10717240893646238No Hit
GATTTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTAC4770.10497174345522085No Hit
TCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGT4750.10453161035897254No Hit
GTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTGCACTTCGCTTC4710.10365134416647594No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4670.10277107797397934No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4610.10145067868523443No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC4610.10145067868523443No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC4580.10079047904086197No Hit
TGATTTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTA4580.10079047904086197No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4570.10057041249273779No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4570.10057041249273779No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG4550.10013027939648951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)