FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454408
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41910.9222989031883242No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA27210.5988010774458197No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC23650.5204573863136213No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA17350.38181546099540503No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCCGG13990.3078731008256897No Hit
GTCTTACATAAGCGGACTCTTGGACTCTCAGCGATGTCAACGACCGACCT13170.28982764387950916No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10810.2378919385222091No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC10600.23327054101160194No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10350.2277688773084981No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG10010.2202866146722769No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9120.2007006918892273No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA8900.19585922783049592No Hit
CCCTTAACTTTATGGGATATATAATTGGAAGTTGGGGTACTTTACCACAG8840.19453882854175103No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTATATATCCCATAAA8500.18705656590552983No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAGGTCTTGCCC8450.18595623316490908No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8330.18331543458741925No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTATATATCCC8240.18133483565430186No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA7960.1751729723068256No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAGGGCATTAAACCTTAT7770.17099170789246668No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7470.1643897114487421No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA7400.16284924561187303No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7400.16284924561187303No Hit
GTACGGGACCATGCAAGACCTGCACGATACCTGCTCAAGGAACCTCTATG7290.16042851358250734No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG7260.15976831393813487No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTGCACTTCGCTTCAC7180.15800778155314169No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC7110.1564673157162726No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7070.155587049523776No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC7020.1544867167831552No Hit
GTCCAAGAGTCCGCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6990.15382651713878276No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT6960.1531663174944103No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGCGGCCG6930.15250611785003787No Hit
GGTCTATTTACAGGAAGTTTTCGAAAGCATTGCTTGATTTTCAGTACAAT6930.15250611785003787No Hit
ATATAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACTGAAA6830.15030545236879633No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6830.15030545236879633No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6770.14898505308005142No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6760.14876498653192727No Hit
CTCTTGGACTCTCAGCGATGTCAACGACCGACCTTGAGGCATACTTCAAA6640.14612418795443743No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGTCTTATAAGTTGGCGA6590.14502385521381667No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6490.14282318973257513No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC6330.1393021249625887No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCT6320.13908205841446453No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6200.1364412598369747No Hit
ATATTGTACTGAAAATCAAGCAATGCTTTCGAAAACTTCCTGTAAATAGA6180.1360011267407264No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTGTGTTTAAAGACT6150.13534092709635395No Hit
CTTTCGTCCCCTTCTGCTTCTGCCGTTCCGGCCGACCACGGGGCGCACCT6060.13336032816323656No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA6010.1322599954226158No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTTCGAAAGC5800.1276385979120086No Hit
CAGTTATATGGATGATTTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5690.12521786588264291No Hit
ACCTTGAGGCATACTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTG5650.1243375996901463No Hit
CAATAGGTCTATTTACAGGAAGTTTTCGAAAGCATTGCTTGATTTTCAGT5610.12345733349764969No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5580.12279713385327723No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5560.12235700075702892No Hit
GTCTTATAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTATACATGC5500.121036601468284No Hit
GGAACATATTGTACTGAAAATCAAGCAATGCTTTCGAAAACTTCCTGTAA5500.121036601468284No Hit
CTGTAAGAGGGCCTACATAGTTTTGACATCTATTAATAATGTCTTCCTGC5350.11773560324642171No Hit
GTGGTAAAGTACCCCAACTTCCAATTATATATCCCATAAAGTTAAGGGAG5270.11597507086142851No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG5260.11575500431330434No Hit
CCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAAGAGTCCGCTTATGT5200.11443460502455942No Hit
GCTTTTCTGTGTTGGGGTGAGTTGATGAATTTGGCCACCTGGGTGGGAAG5190.11421453847643527No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5190.11421453847643527No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTAGGTTAATGATC5180.11399447192831114No Hit
GTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTGCACTTCGCTTC5180.11399447192831114No Hit
TTCTACTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5090.11201387299519376No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAGGGCATTAAAC5080.11179380644706959No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4990.10981320751395221No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG4910.10805267512895901No Hit
ATTTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4900.10783260858083484No Hit
GATTTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTAC4870.10717240893646238No Hit
CCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGAAGTATGCCTCA4840.10651220929208992No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4840.10651220929208992No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4720.10387141071460008No Hit
GTCTATTTACAGGAAGTTTTCGAAAGCATTGCTTGATTTTCAGTACAATA4670.10277107797397934No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4560.10035034594461364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)