FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442922
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18580.4194869525559805No Hit
ATCCTAATGTTACCAAATATTTGCCCTTAGATAAAGGAATTAAACCTTAT17480.39465188001499135No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14180.3201466623920239No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12110.273411571337617No Hit
TCTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10870.24541567138232015No Hit
CATTAGGATAGAACCTAGCGGGCATGATCAATTTTAGTCTCCTGTTTTCA10700.24157752380780362No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10500.23706205607307834No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG10230.23096617463119914No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10000.2257733867362651No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC9720.21945173190764966No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT9620.217193998040287No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8970.2025187279024298No Hit
GGATAATAAGGTTTAATTCCTTTATCTAAGGGCAAATATTTGGTAACATT8690.19619707307381434No Hit
TTATTATCCAGAGCACGTAGTTAATCATTACTTTCAGACGAGACATTATT8340.18829500453804507No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGAAAGTAATGATTAACTACG8290.18716613760436376No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGCTACTCCCTTAACTTCAT8230.18581149728394616No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA8230.18581149728394616No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAAAGAACGACAGAAT8170.18445685696352856No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATAACCACATTGTGTGAAA7890.17813520213491313No Hit
GTATGTAAATAATGTCTCGTCTGAAAGTAATGATTAACTACGTGCTCTGG7880.1779094287481769No Hit
TACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7730.1745228279471329No Hit
GGGTAAAGGTGCAGATATTGTTTGTTTAGAAAGGCCTTATACGTTGGCGA7610.17181354730629772No Hit
TCATTAATAGGTGTAAACAATTTGTGGGACCCCTCACAGTGAATGAAAAC7550.17045890698588012No Hit
GTAGTAAGCTATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTATT7440.16797539973178122No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACGTATAAGGCC7420.16752385295830868No Hit
GTATAAGGCCTTTCTAAACAAACAATATCTGCACCTTTACCCCGTTGCTC7240.1634599319970559No Hit
GTTCTATCCTAATGTTACCAAATATTTGCCCTTAGATAAAGGAATTAAAC7080.15984755780927568No Hit
GCCTTATACGTTGGCGAGAAAGTAAAAGCCTGTTTCGCTTGTATACATGC7020.15849291748885808No Hit
CCTATTAATGATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6900.1557836368480229No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6860.15488054330107784No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6850.15465476991434157No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6740.15217126266024267No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6680.15081662233982507No Hit
ATTAATAGGTGTAAACAATTTGTGGGACCCCTCACAGTGAATGAAAACAG6660.15036507556635256No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6650.15013930217961627No Hit
GTTGTGAGAATGCCCTGAGCTTGAGGGCTCCACCCCAACAGTCCCCCATG6630.14968775540614376No Hit
CTCGTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6460.14584960783162723No Hit
ATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC6370.14381764735100086No Hit
GTAGTCAGCTATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTATT6150.13885063284280302No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6140.13862485945606676No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTAGC6100.1377217659091217No Hit
GGATAGAACCTAGCGGGCATGATCAATTTTAGTCTCCTGTTTTCATTCAC6010.13568980542849532No Hit
AGTATACCCTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTC5950.13433516510807772No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5840.1318516578539788No Hit
ACACATAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAGAGCT5830.13162588446724255No Hit
GTATGGATCGGTAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5810.13117433769377No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGAA5800.13094856430703375No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5750.12981969737335242No Hit
GTGTGAAAGGAGCAGCGAATCCCAAAAGACCCACAATTCGTTGACACACC5740.12959392398661615No Hit
GCTACGTGTTGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTAT5600.12643309657230845No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTTCCAATTTTCTT5500.1241753627049458No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5450.12304649577126446No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATCTGGTTTTGGGGGC5360.12101453529063809No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5350.12078876190390181No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5340.12056298851716556No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5330.12033721513042929No Hit
AGTTAAGGGAGTAGCCCCATCTTTTTGTTTTATGAGGGTTTAAATGTATA5190.11717638771612157No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5190.11717638771612157No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTC5170.11672484094264904No Hit
GTAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAG5110.11537020062223145No Hit
GGGTATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGC5100.11514442723549519No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5100.11514442723549519No Hit
GTTTACAGGAAGTTTTCTAAAACATTGTTTGATTTTCAACACAATATGTT5090.11491865384875893No Hit
ACCCTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5090.11491865384875893No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATTGTTTGATTTTCAAC5060.11424133368855013No Hit
TATTAATGATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTTGC5040.1137897869150776No Hit
CCTGTAAACAGGCCTATTGATTGGAAGGTGTGTCAACGAATTGTGGGTCT5020.11333824014160507No Hit
GGAACATATTGTGTTGAAAATCAAACAATGTTTTAGAAAACTTCCTGTAA4970.11220937320792375No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG4950.11175782643445122No Hit
CCTTCCAATCAATAGGCCTGTTTACAGGAAGTTTTCTAAAACATTGTTTG4890.11040318611403363No Hit
TGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4880.11017741272729736No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG4880.11017741272729736No Hit
ACTTATTGTCCTCCGATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCG4870.10995163934056108No Hit
TTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4850.10950009256708856No Hit
CCTTATGAGTCCAGGGTATACTAACATTGAGATTCCCGAGATTGAGATCT4830.10904854579361603No Hit
GTTCAGAATACTGCCTCTTCCATATCGTCAATCTTATCGAAGACTGGGGA4830.10904854579361603No Hit
GGATAACCACATTGTGTGAAAGGAGCAGCGAATCCCAAAAGACCCACAAT4800.10837122563340723No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACGG4790.10814545224667098No Hit
TAACCACATTGTGTGAAAGGAGCAGCGAATCCCAAAAGACCCACAATTCG4650.10498462483236326No Hit
CTCCTCTACCGATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGC4640.10475885144562701No Hit
CATATAAAGGCATTAAAGCAGGATAACCACATTGTGTGAAAGGAGCAGCG4640.10475885144562701No Hit
CCTTTCACACAATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTAT4640.10475885144562701No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4600.10385575789868193No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4580.10340421112520941No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4550.10272689096500062No Hit
GATTAACTACGTGCTCTGGATAATAAGGTTTAATTCCTTTATCTAAGGGC4530.10227534419152808No Hit
GTTAGTATACCCTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC4480.10114647725784674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)