FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397146
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19860.5000679850734994No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA19470.49024791890136127No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC13720.3454648920044518No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATCTCCTCCCC12530.3155011003510044No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA11670.2938465954585971No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG11350.28578910526607343No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT10580.2664007694903134No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10250.25809148272927335No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGTTTGAGTTTTAGTACAATA10240.25783968616075703No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGGCCCACAATTCTTTGACATACT10180.2563289067496588No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9730.24499806116642242No Hit
CTCCTAATCTCCTCCCCTAACTCCTCCCAGTCTTTAAACACACAGTCTTT9230.23240823274060424No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8760.2205737940203351No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8080.20345162736122233No Hit
CTTCATCTACCGTTCCGACCGACCACGGGGCGCACCTCTCTTTACGCGGT8050.2026962376556732No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG7930.19967467883347687No Hit
GTAGACGAAGAAGGGGACGGTAGAGTCCCAAACGGCCCCGAGACGGGTCG7840.1974085097168296No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7530.18960281609282228No Hit
GTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7490.18859562981875683No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7340.18481868129101137No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7270.18305610531139682No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7170.18053813962623316No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7050.17751658080403682No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGTTACATAAGAGGACTCTTG6910.1739914288448077No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6880.1732360391392586No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6860.1727324460022259No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGTTTGAGTTTTAGTACAAT6670.16794831120041495No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6650.16744471806338224No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6650.16744471806338224No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6590.16593393865228406No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6390.16089800728195675No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT6360.16014261757640766No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGA6310.15888363473382586No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6260.15762465189124403No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC6220.15661746561717857No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6220.15661746561717857No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6170.15535848277459674No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC6120.1540994999320149No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6080.15309231365794948No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6050.1523369239524004No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5960.1500707548357531No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5940.1495671616987204No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA5830.14679739944504036No Hit
TTCCATGGCTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5810.14629380630800765No Hit
CTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCTAACTCCTCCCAG5800.14604200973949127No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5760.14503482346542582No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5750.14478302689690944No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5610.14125787493768036No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5570.1402506886636149No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5520.13899170582103307No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA5510.1387399092525167No Hit
GTCCAAGAGTCCTCTTATGTAACTGAGAGTCCAAGAGTCCTCTTATGTAA5510.1387399092525167No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5460.13748092640993487No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5430.13672553670438578No Hit
CCCCTAACTCCTCCCAGTCTTTAAACACACAGTCTTTGAAGTATGCCTCA5380.13546655386180395No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC5370.1352147572932876No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5350.13471116415625486No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT5340.13445936758773852No Hit
TTATTAATAGATGTCAACAATACGTGGGCCCTCTTACAATTAATGAAAAA5300.13345218131367306No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGTTTGAGTTTTAGT5180.1304306224914767No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG5180.1304306224914767No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACTAAAA5170.13017882592296032No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5120.12891984308037852No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5100.12841624994334577No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT5100.12841624994334577No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5070.1276608602377967No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4960.12489109798411667No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4950.12463930141560031No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4910.12363211514153484No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4880.12287672543598575No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4870.1226249288674694No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA4840.12186953916192031No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4840.12186953916192031No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4810.12111414945637121No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG4790.12061055631933848No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4760.1198551666137894No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4730.11909977690824028No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG4720.11884798033972392No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4690.11809259063417483No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTACCGTTCCGACCGACCACG4660.11733720092862575No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4620.11633001465456028No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4580.11532282838049483No Hit
GGAACATATTGTACTAAAACTCAAACAATGTTTTCGAAAATTGCCTGTAA4570.11507103181197847No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4550.11456743867494573No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT4530.114063845537913No Hit
ATTTACAGGCAATTTTCGAAAACATTGTTTGAGTTTTAGTACAATATGTT4510.11356025240088027No Hit
ATATTGTACTAAAACTCAAACAATGTTTTCGAAAATTGCCTGTAAATAGA4510.11356025240088027No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4510.11356025240088027No Hit
GCACGGGGCCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4470.11255306612681483No Hit
GCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGAT4470.11255306612681483No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4440.11179767642126573No Hit
ACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTT4430.11154587985274936No Hit
CAATTACATATCCCATAAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA4410.11104228671571664No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG4370.11003510044165117No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4330.10902791416758573No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC4300.10827252446203664No Hit
CCTAATCTCCTCCCCTAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA4290.10802072789352027No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTT4290.10802072789352027No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC4280.10776893132500391No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4280.10776893132500391No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT4270.10751713475648753No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4260.10726533818797117No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC4250.10701354161945481No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC4210.10600635534538935No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGG4200.105754558776873No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4150.10449557593429117No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT4150.10449557593429117No Hit
GTACTAAAACTCAAACAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT4150.10449557593429117No Hit
CTATATAAGAGAGAAACTACACGTAGCGCCTCATTTTGTGGGTCACCATA4090.10298479652319298No Hit
ATGTAACTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACC4080.10273299995467661No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4060.10222940681764389No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4020.10122222054357843No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC4020.10122222054357843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)