FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350514
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13580.387431029859007No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT13570.38714573454983253No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA12330.35176911621219126No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT12300.35091323028466764No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT10930.31182777292775754No Hit
CCTCTTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA10390.2964218262323331No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9290.26503934222313513No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA8080.23051860981301744No Hit
TGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7540.21511266311759303No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7540.21511266311759303No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA7400.21111852878914963No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTGATTATGCCTGCTAGG7380.21054793817080059No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG7270.2074096897698808No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7220.2059832132240082No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7220.2059832132240082No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7190.20512732729648459No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT7060.20141848827721576No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6830.19485669616620163No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC6790.1937155149295035No Hit
ACTCACAACTGTGCCAGCGGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6680.19057726652858373No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6600.18829490405518753No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6530.18629783689096585No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTCTGTACAATATGTT6490.18515665565426775No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6310.18002134008912626No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6190.17659779637903195No Hit
CGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCT6110.17431543390563572No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC5630.16062125906525845No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT5590.15948007782856036No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTCTGT5590.15948007782856036No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5540.15805360128268772No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC5440.15520064819094243No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5270.15035062793497547No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5080.1449300170606595No Hit
GGGTAAAGGTTCAGATACTGTTTGCTTAGAAAGGCCTTGTAAGTTGGCGA5070.14464472175148496No Hit
GAAATGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG5060.14435942644231042No Hit
GGAACATATTGTACAGAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4970.1417917686597397No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG4960.14150647335056518No Hit
ATATGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4840.13808292964047086No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4790.13665645309459823No Hit
AGACTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4790.13665645309459823No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT4750.1355152718579001No Hit
TCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTG4740.13522997654872557No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC4690.13380350000285296No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4660.13294761407532937No Hit
GTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA4630.1320917281478058No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAGAAA4590.1309505469111077No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4590.1309505469111077No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAACTCAGA4560.1300946609835841No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4530.12923877505606052No Hit
CCGCATCTGCTTTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCA4500.12838288912853693No Hit
GAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGC4480.1278122985101879No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA4460.12724170789183883No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC4410.1258152313459662No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4390.12524464072761715No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4370.12467405010926812No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC4280.12210639232669736No Hit
CCCCTAACTCCTCCCACTCAGTAAACACACAGTCTTTGAAGTATGCCTCA4280.12210639232669736No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4250.12125050639917379No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4190.11953873454412663No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4150.11839755330742852No Hit
CCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTT4120.11754166737990494No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4100.11697107676155588No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA4100.11697107676155588No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4050.11554460021568325No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA4050.11554460021568325No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4040.11525930490650872No Hit
GGCCAAGACTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATT4030.1149740095973342No Hit
GCTCACGGAACCTCTATGTTTCCCTCATGTTGCTGTACGAAACCTACGGA4020.11468871428815969No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC3980.11354753305146156No Hit
CATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGCTT3980.11354753305146156No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3970.11326223774228703No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC3960.11297694243311253No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGACTGTACAACATCTTG3860.11012398934136725No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG3840.1095533987230182No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT3820.10898280810466915No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3810.10869751279549461No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT3800.10841221748632009No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT3780.10784162686797104No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3780.10784162686797104No Hit
GGATAAAACCTAGCAGGCATAATCAATTTTAGTCTCCTGTTTTCATTTAC3750.10698574094044745No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3740.10670044563127293No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC3730.10641515032209842No Hit
GTACCATAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3710.10584455970374936No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3710.10584455970374936No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG3680.10498867377622577No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGACTGTACAACATCTTGAG3640.10384749253952766No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCCGGGGGCGTTCACGGTGGTCTC3640.10384749253952766No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3600.10270631130282956No Hit
ATATTGTACAGAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAAACAGG3590.10242101599365504No Hit
TTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTT3580.1021357206844805No Hit
GCTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAG3530.10070924413860788No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)