FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350514
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT13000.3708839019268845No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12670.36146915672412516No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA11340.32352488060391316No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10920.311542477618583No Hit
CCTCTTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA10290.2935688731405878No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT9710.2770217452084653No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9640.2750246780442436No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8010.22852154264879576No Hit
TGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7680.2191067974460364No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7680.2191067974460364No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTGATTATGCCTGCTAGG7660.21853620682768735No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG7660.21853620682768735No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7500.2139714818808949No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7230.2062685085331827No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7120.20313026013226287No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7000.19970671642216856No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC6770.1931449243111545No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTCTGTACAATATGTT6680.19057726652858373No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT6540.18658313220014036No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6390.18230370256252246No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA6340.18087722601664982No Hit
ACTCACAACTGTGCCAGCGGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6340.18087722601664982No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6060.1728889573597631No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5980.17060659488636687No Hit
CGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCT5970.17032129957719233No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5820.16604186993957445No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT5580.15919478251938582No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC5530.15776830597351318No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5520.15748301066433865No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC5510.15719771535516414No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5470.15605653411846604No Hit
ATATGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5350.1526329904083717No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5300.1512065138624991No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4970.1417917686597397No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG4950.14122117804139064No Hit
GGGTAAAGGTTCAGATACTGTTTGCTTAGAAAGGCCTTGTAAGTTGGCGA4940.1409358827322161No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC4900.139794701495518No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4870.13893881556799442No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTCTGT4860.13865352025881988No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4860.13865352025881988No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4850.13836822494964537No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4810.13722704371294728No Hit
GGAACATATTGTACAGAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4780.1363711577854237No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC4760.13580056716707464No Hit
AGACTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4680.13351820469367842No Hit
GTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA4610.13152113752945674No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA4520.128953479746886No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC4510.12866818443771147No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4450.1269564125826643No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4440.12667111727348979No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4410.1258152313459662No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT4400.1255299360367917No Hit
GGCCAAGACTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATT4400.1255299360367917No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA4290.1223916876358719No Hit
GAAATGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG4270.12182109701752283No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA4240.12096521108999926No Hit
CCGCATCTGCTTTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCA4210.12010932516247567No Hit
GTACCATAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4150.11839755330742852No Hit
GAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGC4140.118112257998254No Hit
CCCCTAACTCCTCCCACTCAGTAAACACACAGTCTTTGAAGTATGCCTCA4090.11668578145238136No Hit
ATATTGTACAGAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAAACAGG4060.11582989552485778No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGACTGTACAACATCTTGAG4030.1149740095973342No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4020.11468871428815969No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4000.11411812366981063No Hit
TCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTG4000.11411812366981063No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC3990.11383282836063609No Hit
GTCTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGACAGTG3990.11383282836063609No Hit
CATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGCTT3980.11354753305146156No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT3980.11354753305146156No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAACTCAGACA3960.11297694243311253No Hit
GGATAAAACCTAGCAGGCATAATCAATTTTAGTCTCCTGTTTTCATTTAC3930.11212105650558893No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT3920.1118357611964144No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3910.11155046588723987No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC3880.11069457995971631No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT3880.11069457995971631No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3860.11012398934136725No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA3830.10926810341384367No Hit
TTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTT3800.10841221748632009No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3780.10784162686797104No Hit
CCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTT3770.10755633155879651No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAGAAA3700.10555926439457482No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAACTCAGA3700.10555926439457482No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3670.10470337846705126No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3620.10327690192117861No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3590.10242101599365504No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG3580.1021357206844805No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTG3580.1021357206844805No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC3550.10127983475695694No Hit
GCTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAG3510.10013865352025882No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)