FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483046
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG28790.5960094897794412No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18110.37491253420999243No Hit
ATCCTAACCTTACTAAATATTTGCCCTTGGACAAAGGCATTAAACCCTAT17060.353175473971423No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG14160.2931397837887075No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA13380.2769922533257702No Hit
GTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT12810.26519213491054683No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA11550.23910766262426353No Hit
GTTCTATCCTAACCTTACTAAATATTTGCCCTTGGACAAAGGCATTAAAC11230.23248303474203286No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10860.22482330875320364No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10450.21633550427909556No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10370.2146793473085379No Hit
GGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTAGTAAGGTT9350.19356334593392763No Hit
CTTATATAGAATGCCGGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9170.18983699275017285No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8760.1813491882760648No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT8720.18052110979078598No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8610.17824389395626916No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8450.17493158001515383No Hit
GTGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8440.17472456039383413No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGGCCTAT8270.17120522683139908No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG7750.16044020652277421No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7650.15837001030957715No Hit
CCTCCTGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA7640.15816299068825743No Hit
GTTTCAGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACA7460.15443663750450268No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7380.152780480533945No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7380.152780480533945No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA7160.14822604886491142No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7140.147812009622272No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6830.14139440136136103No Hit
GTTCAGGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTAGTA6610.13683996969232745No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA6600.13663295007100773No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAGGGTTTAATGCC6540.13539083234308946No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6460.1337346753725318No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6240.1291802437034982No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6230.1289732240821785No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6210.12855918483953907No Hit
CTGACATACTTTCCAATCAATAGGCCTATTTACAGGCAGTTTTCGAAAAC6120.1266960082476617No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6090.1260749493837026No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5990.1240047531705055No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5970.1235907139278661No Hit
GTACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGGCCTAT5800.12007138036543104No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5630.11655204680299598No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAAT5590.11572396831771715No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT5440.11261867399792153No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG5430.11241165437660182No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC5390.11158357589132296No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC5360.11096251702736384No Hit
ATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5290.10951337967812588No Hit
CTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5170.10702914422228939No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5140.10640808535833025No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTTTGGTTTCACATTTCC5130.10620106573701056No Hit
ATTTTGTGGTCTGTAAGCAGGAGGAGTGCGAATCCACACTCCGAAAGACA5030.10413086952381348No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4990.10330279103853462No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTGTGT4980.10309577141721493No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA4980.10309577141721493No Hit
CTCCGAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4960.1026817321745755No Hit
CTCTTATATAGAATGCCGGCCTTCCACAGAGTATGTAAATAATGCCTAGT4940.10226769293193609No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT4910.10164663406797697No Hit
GCCCAAAAGACCCACAATTCTCTGACATACTTTCCAATCAATAGGCCTAT4890.10123259482533754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)