FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483046
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG31510.652318826778402No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18040.37346339686075447No Hit
ATCCTAACCTTACTAAATATTTGCCCTTGGACAAAGGCATTAAACCCTAT16250.3364068846445266No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG13770.2850660185572389No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA13470.2788554299176476No Hit
GTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT12820.2653991545318665No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA12460.257946448164357No Hit
GTTCTATCCTAACCTTACTAAATATTTGCCCTTGGACAAAGGCATTAAAC11810.24449017277857593No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11070.2291707208009175No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10700.22151099481208827No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10560.21861272011361238No Hit
CTTATATAGAATGCCGGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10280.2128161707166605No Hit
GGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTAGTAAGGTT9710.20101605230143713No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8970.1856966003237787No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8460.17513859963647355No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGGCCTAT8380.1734824426659159No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT8280.1714122464527188No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG7950.1645805989491684No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7900.16354550084256986No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7770.16085424576541366No Hit
GTTTCAGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACA7570.15671385333901947No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA7470.1546436571258224No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAGGGTTTAATGCC7160.14822604886491142No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7100.14698393113699318No Hit
CCTCCTGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA7070.14636287227303404No Hit
GTGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6910.14305055833191868No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6880.14242949946795957No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6710.13891016590552452No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6610.13683996969232745No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6270.12980130256745734No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6210.12855918483953907No Hit
GTTCAGGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTAGTA6190.12814514559689968No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA6120.1266960082476617No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6100.1262819690050223No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5990.1240047531705055No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5970.1235907139278661No Hit
GTACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGGCCTAT5970.1235907139278661No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5840.12089945885070986No Hit
CTCTTATATAGAATGCCGGCCTTCCACAGAGTATGTAAATAATGCCTAGT5840.12089945885070986No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC5760.11924330188015221No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5590.11572396831771715No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAAT5550.11489588983243833No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT5510.11406781134715947No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC5310.10992741892076531No Hit
CTGACATACTTTCCAATCAATAGGCCTATTTACAGGCAGTTTTCGAAAAC5290.10951337967812588No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5260.10889232081416676No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5190.10744318346492882No Hit
GTCTTACACAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5170.10702914422228939No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG5120.10599404611569085No Hit
GATTAACTGCATGTTCAGGATAATAGGGTTTAATGCCTTTGTCCAAGGGC5050.10454490876645288No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5050.10454490876645288No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG5050.10454490876645288No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTTTGGTTTCACATTTCC5040.10433788914513317No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT5020.10392384990249376No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC4920.10185365368929668No Hit
GCCCAAAAGACCCACAATTCTCTGACATACTTTCCAATCAATAGGCCTAT4910.10164663406797697No Hit
CTCCGAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4900.10143961444665725No Hit
CTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4890.10123259482533754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)