FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561894
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC24710.43976265986111257No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT22950.40844002605473634No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC22590.40203312368525024No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA22360.3979398249491897No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT18980.3377861304801261No Hit
ATCACGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC16820.29934471626320974No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA16570.29489547850662223No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG15010.267132234905516No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG14920.2655305093131445No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC14860.2644626922515635No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG14690.2614372105770839No Hit
CTCCAAAAGAGACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA13780.24524198514310527No Hit
TACCGTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG12740.2267331560757011No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG12370.22014828419595153No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG12010.21374138182646546No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT11600.20644463190566195No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG11440.2035971197414459No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC11200.1993258514951219No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG10810.1923850405948453No Hit
GCCTAGCAGCCATGGGAAGGATGTATACTTGCGGGAGAGCACGACAGAAT10780.1918511320640548No Hit
ATATGCAGGGGGAGTGCGAATCCACACTCCAAAAGAGACCAAATATTCAA10670.18989346745115626No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC10320.18366453459193371No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC10220.18188483948929868No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT10190.18135093095850818No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG9890.17601184565060313No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG9140.16266413238084051No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA9100.16195225433978652No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC9010.160350528747415No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8920.15874880315504347No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8870.15785895560372598No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA8850.15750301658319896No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8740.15554535197030045No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCTCTTAACTTCAT8520.1516300227445034No Hit
CAATGACATATCCCATGAAGTTAAGAGAATATCCCCATCTTTTTGTTTTA8470.1507401751931859No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT8340.14842657155976038No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATATGGGAGTCGGCTGCCT8160.14522312037501733No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8090.14397733380317285No Hit
GAGGCAGATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAA7820.13917215702605829No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGG7810.1389941875157948No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT7710.13721449241315978No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC7700.13703652290289628No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA7660.13632464486184226No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT7530.13401104122841676No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7400.13169743759499122No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7260.1292058644513022No Hit
GTATACATCCTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC7250.1290278949410387No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA7240.1288499254307752No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG7180.1277821083691942No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7060.12564647424603217No Hit
CCCATATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7050.12546850473576868No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC6940.12351084012287014No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATGACATATCCCATGAA6930.12333287061260666No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6920.12315490110234315No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG6890.12262099257155264No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC6840.12173114502023513No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTTCTCCTGC6750.12012941942786362No Hit
GTCTATATGCAGGGGGAGTGCGAATCCACACTCCAAAAGAGACCAAATAT6680.11888363285601909No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6680.11888363285601909No Hit
GTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGTC6520.11603612069180308No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6510.11585815118153957No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6390.11372251705837756No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6350.11301063901732356No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGC6340.11283266950706004No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG6340.11283266950706004No Hit
AGATTCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTC6340.11283266950706004No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6310.11229876097626953No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6290.11194282195574254No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATT6290.11194282195574254No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC6290.11194282195574254No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6270.11158688293521554No Hit
CTTTTGGAGTGTGGATTCGCACTCCCCCTGCATATAGACCACAAAATGCC6250.11123094391468855No Hit
CGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAA6190.11016312685310753No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC6160.10962921832231702No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6040.107493584199155No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6010.10695967566836449No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAC5930.10553591958625648No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5910.10517998056572947No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5850.10411216350414848No Hit
GACATATCCCATGAAGTTAAGAGAATATCCCCATCTTTTTGTTTTATGAG5850.10411216350414848No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT5830.10375622448362146No Hit
CTACCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC5760.10251043791177696No Hit
CAATTACATATCCCATGAAGTTAAGAGAATATCCCCATCTTTTTGTTTTA5730.10197652938098645No Hit
GGTCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC5670.10090871231940544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)