FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419205
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA15410.3676005772831908No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14100.3363509500125237No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT13990.3337269355088799No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13030.31082644529526127No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12400.29579799859257405No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12290.29317398408893025No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC11630.27742989706706744No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG11570.27599861642871626No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT11240.26812657291778486No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10920.260493076179912No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9360.22327977958278167No Hit
TCCTAACATTCATTTGCAGGAGGACATTGTTGATAGATGTAAGCAATTTG9140.21803175057549407No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA9000.21469209575267473No Hit
GGATAATAAGATTTGATCCCTTTATCTAAGGGCAGATATTTCGTAACATT8780.20944406674538713No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG8180.19513126036187545No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGCACGACAGAAT8010.1910759652198805No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7980.19036032490070492No Hit
TGCAATAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7860.18749776362400258No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7800.18606648298565143No Hit
CCTATTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7720.1841581088011832No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7360.17557042497107622No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG7330.17485478465190063No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7250.17294641046743242No Hit
ATATGTTCCTGAGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7200.17175367660213975No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA7080.16889111532543744No Hit
GTGGATATCCTGCTCTAATGCCTTTGTATGCATGTATACAAGCAAAACAG6840.16316599277203278No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6360.15171574766522344No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGT6310.15052301379993083No Hit
CTATCCGAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCTTTA6200.14789899929628703No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6180.14742190575016997No Hit
GTATCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA6140.14646771865793584No Hit
TTTCCAGGGGGAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAAT6120.14599062511181882No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC6040.14408225092735058No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA6020.14360515738123353No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6010.14336661060817502No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5930.14145823642370678No Hit
GGAAGATACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT5900.14074259610453121No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCGACTTACAAGGCC5810.13859567514700444No Hit
GTATACATGCATACAAAGGCATTAGAGCAGGATATCCACATTGCGTAAAA5790.1381185816008874No Hit
AAAACTCAGACAAATATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGA5730.13668730096253623No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA5650.13477892677806802No Hit
GTATCGGGAGGCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5620.13406328645889243No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT5600.13358619291277538No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5580.13310909936665832No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5570.1328705525935998No Hit
CGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCT5540.13215491227442422No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5500.13120072518219011No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5480.13072363163607303No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTGCTT5390.1285767106785463No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT5340.12738397681325367No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGGACGTCCTTTGTCTA5240.12499850908266838No Hit
TAGATAAAGGGATCAAATCTTATTATCCAGAGCATGTAGTTAATCATTAC5240.12499850908266838No Hit
CCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTGTGGTATTG5210.1242828687634928No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT5160.12309013489820017No Hit
ATGTCAGGAAAAGAAGGAGTTTGCCATTTAGGATTAAAGCAAGGTACCGT5140.1226130413520831No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5130.12237449457902458No Hit
CTTTATCTAAGGGCAGATATTTCGTAACATTGGGATAAAACCTAGCAGGC5130.12237449457902458No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5120.12213594780596607No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5110.12189740103290753No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCGGATTGGGACCT5090.12142030748679047No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5020.11975048007538078No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5010.11951193330232227No Hit
ATGCAATAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA4930.11760355911785403No Hit
TAGCCGACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCAC4890.11664937202561992No Hit
CCCTCAGAGGTGGAGATATGGGAGTAGGCTGCCTTCCTGACTGCCGATTG4860.11593373170644433No Hit
ACTACATGCTCTGGATAATAAGATTTGATCCCTTTATCTAAGGGCAGATA4840.1154566381603273No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA4840.1154566381603273No Hit
GTCCAACTGATGGTCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4820.11497954461421023No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT4810.1147409978411517No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG4810.1147409978411517No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGACTTGTGGGTCT4770.1137868107489176No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4670.11140134301833232No Hit
GCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4670.11140134301833232No Hit
GACAAATATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACT4650.11092424947221527No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC4640.11068570269915673No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4580.10925442206080557No Hit
GTATAAAACCTACGGACGGCAACTGCACCTGTATTCCCATCCCATCATCT4560.10877732851468852No Hit
CCTCTGAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT4480.1068689543302203No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4460.10639186078410323No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4460.10639186078410323No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4370.1042449398265765No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4360.10400639305351797No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4320.10305220596128387No Hit
CAAATATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGT4310.10281365918822533No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4310.10281365918822533No Hit
GGATAATACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT4300.1025751124151668No Hit
GGATATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAAG4290.10233656564210827No Hit
GCAATAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAAC4290.10233656564210827No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC4250.10138237854987417No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4250.10138237854987417No Hit
GGGTAAAGGTTCAGATACTGTTTGCTTAGAAAGGCCTTGTAAGTCGGCGA4220.10066673823069859No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4210.10042819145764006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)