FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419205
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA14430.3442229935234551No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT13720.32728617263629967No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12290.29317398408893025No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12130.28935723571999383No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11810.28172373898212094No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG11450.27313605515201395No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11390.27170477451366276No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC10650.25405231330733175No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT10170.2426020682005224No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9990.23830822628546894No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9370.2235183263558402No Hit
GGATAATAAGATTTGATCCCTTTATCTAAGGGCAGATATTTCGTAACATT9360.22327977958278167No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8060.19226869908517313No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA8050.1920301523121146No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG8020.19131451199293903No Hit
TCCTAACATTCATTTGCAGGAGGACATTGTTGATAGATGTAAGCAATTTG7920.18892904426235374No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG7880.18797485717011964No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7500.1789100797938956No Hit
GTATCGGGAGGCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7180.17127658305602272No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7130.17008384919073008No Hit
GTATCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA7000.16698274114096923No Hit
TGCAATAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6850.16340453954509132No Hit
GTGGATATCCTGCTCTAATGCCTTTGTATGCATGTATACAAGCAAAACAG6750.16101907181450603No Hit
ATATGTTCCTGAGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6750.16101907181450603No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6720.16030343149533044No Hit
GTATACATGCATACAAAGGCATTAGAGCAGGATATCCACATTGCGTAAAA6580.15696377667251107No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT6560.15648668312639402No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGCACGACAGAAT6540.15600958958027694No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6370.15195429443828198No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6290.15004592025381377No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC6030.14384370415429204No Hit
TTTCCAGGGGGAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAAT5960.14217387674288237No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5940.14169678319676532No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5840.13931131546618003No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGT5810.13859567514700444No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5800.13835712837394593No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGGACGTCCTTTGTCTA5740.13692584773559477No Hit
CCTATTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5710.13621020741641918No Hit
ATGCAATAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA5600.13358619291277538No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA5570.1328705525935998No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5560.13263200582054127No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCGACTTACAAGGCC5550.13239345904748273No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5530.13191636550136568No Hit
CTATCCGAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCTTTA5470.13048508486301452No Hit
GGAAGATACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT5420.12929235099772188No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5420.12929235099772188No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5410.12905380422466334No Hit
GTATAAAACCTACGGACGGCAACTGCACCTGTATTCCCATCCCATCATCT5270.12571414940184397No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT5230.12475996230960987No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTGCTT5210.1242828687634928No Hit
ATGTCAGGAAAAGAAGGAGTTTGCCATTTAGGATTAAAGCAAGGTACCGT5200.12404432199043428No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA5150.12285158812514163No Hit
GTATTATCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA5120.12213594780596607No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT5070.12094321394067341No Hit
CGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCT5040.12022757362149783No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4940.11784210589091257No Hit
CTTTATCTAAGGGCAGATATTTCGTAACATTGGGATAAAACCTAGCAGGC4930.11760355911785403No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT4920.1173650123447955No Hit
AAAACTCAGACAAATATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGA4890.11664937202561992No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4830.11521809138726877No Hit
CCCTCAGAGGTGGAGATATGGGAGTAGGCTGCCTTCCTGACTGCCGATTG4810.1147409978411517No Hit
CCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTGTGGTATTG4790.11426390429503464No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGACTTGTGGGTCT4790.11426390429503464No Hit
GGATAATACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT4760.11354826397585907No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCGGATTGGGACCT4720.11259407688362497No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG4710.11235553011056643No Hit
GTTTTATCCCAATGTTACGAAATATCTGCCCTTAGATAAAGGGATCAAAT4660.1111627962452738No Hit
GCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4640.11068570269915673No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4640.11068570269915673No Hit
GTCCAACTGATGGTCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4560.10877732851468852No Hit
GACAAATATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACT4520.1078231414224544No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC4520.1078231414224544No Hit
ACTACATGCTCTGGATAATAAGATTTGATCCCTTTATCTAAGGGCAGATA4410.1051991269188106No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4400.10496058014575207No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA4370.1042449398265765No Hit
GGGTAAAGGTTCAGATACTGTTTGCTTAGAAAGGCCTTGTAAGTCGGCGA4350.10376784628045943No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG4350.10376784628045943No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4340.10352929950740092No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG4310.10281365918822533No Hit
TAGCCGACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCAC4300.1025751124151668No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4290.10233656564210827No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4270.10185947209599122No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4240.10114383177681563No Hit
GGATATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAAG4220.10066673823069859No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA4200.10018964468458152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)