FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484238
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN94411.9496611170540108No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC20920.43201896588041416No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15940.32917697495859477No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT13420.2771364494318909No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA13030.2690825585765677No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT11430.23604095506754944No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTATCCTCCCC10320.213118342633168No Hit
ATTCAAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA8940.1846199596066397No Hit
CAATTACATATCCCATGAAGTTAAGGGAATAACCCCATCTTTTTGTTTTG8400.17346841842234603No Hit
CCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTC8290.17119680818110103No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8050.1662405676547483No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7140.14744815565899413No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6930.14311144519843547No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6880.14207889508877866No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT6800.14042681491332776No Hit
GTATCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACC6680.1379486946501514No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6160.12721017350972044No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT6140.1267971534658577No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6030.12452554322461269No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5980.12349299311495587No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5960.12307997307109313No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5940.12266695302723041No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5800.1197758127201913No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5720.1181237325447404No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT5670.11709118243508358No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5590.11543910225963265No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCGGTGAGCGTTC5560.11481957219383856No Hit
GCCTAGCAGCCATGGGAATGATGTATACTTGCGGGAGAGCACGACAGAAT5520.11399353210611311No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5510.11378702208418175No Hit
ATCTCGAACAGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGAGTAACTCCA5490.11337400204031903No Hit
GCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCC5460.11275447197452491No Hit
GTCCGGTAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5350.11048286173327992No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA5180.10697219136044672No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5160.106559171316584No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5030.10387454103147627No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5000.10325501096568217No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT4970.10263548089988807No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4890.10098340072443716No Hit
ATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC4850.10015736063671171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)