FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484238
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33130.68416770265861No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC20950.4326384959462083No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA18430.38059797041950444No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT14040.28994007079163553No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA12380.25565940715102903No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTATCCTCCCC11370.23480189493596126No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT11130.2298456544096085No Hit
ATTCAAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA8880.1833808994750515No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8240.1701642580714442No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7490.15467600642659188No Hit
GTATCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACC7300.15075231600989597No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7190.14848070576865097No Hit
CAATTACATATCCCATGAAGTTAAGGGAATAACCCCATCTTTTTGTTTTG6930.14311144519843547No Hit
CCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTC6910.14269842515457273No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6910.14269842515457273No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6910.14269842515457273No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT6860.14166587504491593No Hit
GCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCC6450.13319896414573No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6240.12886225368517135No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT6150.12700366348778908No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6130.12659064344392634No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT6080.1255580933342695No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA6030.12452554322461269No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC6010.12411252318074997No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5980.12349299311495587No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5880.12142789289564222No Hit
GCCTAGCAGCCATGGGAATGATGTATACTTGCGGGAGAGCACGACAGAAT5810.11998232274212269No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5790.11956930269825994No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA5760.11894977263246585No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTGT5570.11502608221576993No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5540.11440655214997585No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA5530.11420004212804448No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5400.11151541184293674No Hit
ATCTCGAACAGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGAGTAACTCCA5400.11151541184293674No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5360.11068937175521129No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5360.11068937175521129No Hit
GTATACATCATTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC5260.10862427153589763No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5250.10841776151396627No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGTTATT5210.10759172142624082No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5200.10738521140430946No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACAATTCCGGA5170.10676568133851537No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC5140.10614615127272126No Hit
CTCCAAAGGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5130.10593964125078989No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5100.1053201111849958No Hit
ATGCAGTAGGAGTGCGAATCCACACTCCAAAGGACACCAAATATTCAAGA5090.10511360116306444No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCGGTGAGCGTTC5060.10449407109727035No Hit
GTCCGGTAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5050.10428756107533899No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5040.10408105105340762No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5040.10408105105340762No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5020.1036680310095449No Hit
CTACCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAAACC5000.10325501096568217No Hit
GTCCAAGAGTCCTCTTATGCAAGACCGAAAGTCCAAGAGTCCTCTTATGC4970.10263548089988807No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT4960.1024289708779567No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4890.10098340072443716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)