FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273517
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA15500.5666923810951421No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9070.331606444937609No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7660.2800557186573412No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7310.2672594390842251No Hit
ATGTAAGACCTTGGGCAAGACTTGGTGGGCGTTCACGGTGGTCTCCATGC6650.24312931188920617No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTCCCTTCTATTCG6620.2420324879257962No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6510.2380108000599597No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6440.23545154414533648No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6160.22521452048684362No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCTACCAATCGGCAGT5650.2065685131088744No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5580.2040092571942512No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5390.19706270542598814No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5290.19340662554795499No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4980.18207277792605212No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4860.17768548207241233No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACTTGGTGGGCGTTC4800.1754918341455924No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG4800.1754918341455924No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT4730.1729325782309692No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4710.17220136225536256No Hit
ATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT4580.16744845841391942No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4540.16598602646270616No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4480.16379237853588624No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4420.16159873060906635No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTGCCGCAG4400.16086751463345972No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4400.16086751463345972No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4400.16086751463345972No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4320.15794265073103317No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4260.15574900280421328No Hit
GTTCCAATAAGTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAG4050.1480712350603436No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4020.14697441109693365No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA3960.14478076317011374No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG3900.14258711524329384No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3880.1418558992676872No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA3830.1400278593286706No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3820.1396622513408673No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC3820.1396622513408673No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGGAA3760.13746860341404737No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT3760.13746860341404737No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3740.13673738743844077No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3740.13673738743844077No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG3730.13637177945063744No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3640.1330813075604076No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC3630.13271569957260426No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG3600.1316188756091943No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT3600.1316188756091943No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3570.13052205164578437No Hit
GTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3570.13052205164578437No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT3550.1297908356701777No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT3530.12905961969457108No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA3470.1268659717677512No Hit
ACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTT3460.12650036377994786No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG3450.12613475579214456No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT3440.12576914780434123No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCCTCCTGT3410.12467232384093128No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG3400.12430671585312796No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC3340.12211306792630806No Hit
GCCCCATCCAGTGGGGGTTGCATCAGCAAACACTTGGCAGAGACCTGACC3300.12065063597509477No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3280.11991941999948816No Hit
CCTCCTTTCCATGGCTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGG3270.11955381201168483No Hit
CCACAATAGTTGTCTGATTTTTAGGCCCATATTAACATTGACATAGCTGA3230.11809138006047155No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA3200.11699455609706161No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT3170.11589773213365165No Hit
GTATGGATCGGCAGGGGAGCCACAAAGGTTCCACGCAGGCGCCGATGGCC3170.11589773213365165No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC3140.11480090817024169No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3140.11480090817024169No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3140.11480090817024169No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3130.11443530018243839No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA3110.11370408420683176No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA3090.11297286823122511No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA3030.11077922030440521No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGG3020.11041361231660189No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT3010.11004800432879859No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAAGTCTTGCCC2980.10895118036538863No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG2970.1085855723775853No Hit
GTTCTATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAAC2970.1085855723775853No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATCTGG2950.10785435640197867No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTTGGCCGGAACGGCAGATGAAGAAGG2930.10712314042637203No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC2930.10712314042637203No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG2930.10712314042637203No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC2930.10712314042637203No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTTGGCCGGAACGG2900.10602631646296208No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGCCCCGTG2890.10566070847515877No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA2890.10566070847515877No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG2880.10529510048735546No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG2870.10492949249955212No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC2850.1041982765239455No Hit
ATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGGAAGAAGT2840.10383266853614218No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA2830.10346706054833886No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA2810.10273584457273222No Hit
CCCCAACACAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATG2800.1023702365849289No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT2790.1020046285971256No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCCTGCGTGGAACCTTTGTGGC2750.10054219664591232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)