FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220944
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8150.3688717503077703No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG6790.3073176913607068No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6640.30052864074154534No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT6110.2765406618871751No Hit
GTATAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC5700.2579839235281338No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5420.2453110290390325No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCACTTAACTTCAT5200.23535375479759577No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT4970.22494387718154824No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4730.21408139619088998No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA4680.2118183793178362No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC4670.21136577594322542No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4580.2072923455717286No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4500.2036715185748425No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4440.20095589832717795No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4250.1923564342095735No Hit
ATATTATACTAAAACTCAAGCAATGTTTTCGCAAATTGCCTGTAAATAGA4200.19009341733651966No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4120.18647259033963356No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA3960.17923093634586137No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3940.17832572959663986No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT3830.1733470924759215No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA3820.17289448910131072No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3800.1719892823520892No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3530.15976899123759866No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA3520.1593163878629879No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA3370.1525273372438265No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT3290.1489065102469404No Hit
CAATAGGTCTATTTACAGGCAATTTGCGAAAACATTGCTTGAGTTTTAGT3270.14800130349771887No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTGCG3240.1466434933738866No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCCGACTTCTTTCCTTCTATTCG3240.1466434933738866No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3150.14257006300238975No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3060.1384966326308929No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC3050.13804402925628212No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3020.13668621913244985No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC3010.1362336157578391No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA3000.13578101238322832No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA2950.13351799551017451No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA2930.132612788760953No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT2910.13170758201173147No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT2900.1312549786371207No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC2880.1303497718878992No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCC2740.12401332464334854No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG2710.12265551451951626No Hit
GTCTATTTACAGGCAATTTGCGAAAACATTGCTTGAGTTTTAGTATAATA2700.1222029111449055No Hit
CACTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG2680.12129770439568398No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC2670.12084510102107321No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG2590.11722427402418713No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA2570.1163190672749656No Hit
ACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTT2560.11586646390035485No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGG2550.11541386052574408No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG2510.11360344702730103No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA2490.11269824027807951No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC2480.11224563690346875No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG2390.1081722065319719No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA2390.1081722065319719No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA2370.10726699978275038No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC2330.10545658628430735No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG2280.10319356941125353No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC2260.102288362662032No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC2250.10183575928742125No Hit
TCCTCACATTCATTTACAGGAGGACATTATTAATAGATGTCAACAATATG2250.10183575928742125No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA2250.10183575928742125No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG2210.1000253457889782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)