FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424182
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20460.48234012758674344No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC18760.44226299088598764No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG16040.37813957216477834No Hit
ATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGT15340.36163722175858476No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15020.35409329014432483No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14620.34466337562649996No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13620.3210885893319377No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13050.3076509611440372No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA11450.26993130307273766No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10580.2494212389964685No Hit
GTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCTTTGACATACT10210.24069856806748047No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9860.23244739286438368No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGACGA9670.22796818346841685No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATGTCCTCCTGT8810.20769386725509334No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8510.20062143136672467No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8430.1987354484631597No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8200.19331324761541038No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA8070.19024852539711729No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTAAC7870.18553356813820482No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7820.18435482882347673No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7800.18388333309758548No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC7490.17657514934627117No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7480.17633940148332555No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7470.17610365362037994No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7410.17468916644270618No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7390.17421767071681496No Hit
CTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTC7160.16879546986906563No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAA7000.16502350406193567No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6870.1619587818436426No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6860.16172303398069696No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6770.15960130321418636No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6750.15912980748829514No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCA6660.15700807672178452No Hit
ACTCCAGATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAA6660.15700807672178452No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6550.15441485022938267No Hit
GTGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC6530.15394335450349142No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA6490.15300036305170894No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGTCAACTTACAAGGCC6490.15300036305170894No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6490.15300036305170894No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTCTTCTACTG6490.15300036305170894No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6460.15229311946287205No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6360.14993564083341585No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6300.14852115365574212No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTGTGT6270.14781391006690522No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG6250.147342414341014No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5960.14050572631559094No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA5940.14003423058969972No Hit
CTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTCCT5840.13767675196024348No Hit
CTCTAAGGCCTCCCGATAGAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5720.13484777760489602No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCA5700.13437628187900474No Hit
GCTTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGT5660.13343329042722227No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5630.1327260468383854No Hit
GGCCTACATATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATG5630.1327260468383854No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5620.1324902989754398No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAACCTG5610.13225455111249418No Hit
GGAACATATTGTACAAAAACTTAAGCAATGTTTTCGGAAACTGCCTGTAA5590.13178305538660293No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5590.13178305538660293No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5570.13131155966071167No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG5550.13084006393482042No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5550.13084006393482042No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAAT5530.1303685682089292No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5490.1294255767571467No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5440.12824683744241858No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5400.1273038459906361No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5340.12588935881296237No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5320.12541786308707112No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5300.12494636736117987No Hit
ACATATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAGG5290.12471061949823425No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5280.12447487163528863No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT5240.12353188018350615No Hit
GTGAAAGCCTGCTTAGCTTGTATACATGCATATAAAGGCATCAAAGCAGG5180.12211739300583241No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTACTCTTCTACTGTACCTGTC5100.12023141010226743No Hit
CCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAACC5040.11881692292459368No Hit
GTGTTGGGGTGAGTTGATGAACCTGGCCACCTGGGTGGGAAGTAATTTGG4990.11763818360986558No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4930.11622369643219184No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT4930.11622369643219184No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4840.11410196566568123No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4800.11315897421389876No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4750.11198023489917064No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4740.11174448703622501No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4710.11103724344738815No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4670.11009425199560567No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4650.1096227562697144No Hit
ATTGACACCAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4650.1096227562697144No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT4650.1096227562697144No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4630.10915126054382317No Hit
GCAAATCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGG4620.10891551268087755No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4560.10750102550320381No Hit
CTTTACCACAGGAACATATTGTACAAAAACTTAAGCAATGTTTTCGGAAA4530.10679378191436695No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC4500.10608653832553008No Hit
CTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4470.1053792947366932No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCT4460.10514354687374758No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG4460.10514354687374758No Hit
GTAGAAGAGTAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT4440.10467205114785634No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4420.1042005554219651No Hit
GTCCAAGAGTCCTCTTGTGTAAGACCTTGGGCAAGACCTGATGGGCGTTC4370.10302181610723699No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC4360.10278606824429136No Hit
GGTTATTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCC4280.10090008534072638No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4260.10042858961483514No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4260.10042858961483514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)