FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424182
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20290.47833241391666786No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG17230.4061935678553074No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16840.39699940120042815No Hit
ATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGT16020.3776680764388871No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14080.33193299102743634No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13400.315902136347134No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT12150.28643365347893124No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10830.2553149355701091No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA10630.2505999783111966No Hit
GTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCTTTGACATACT10380.24470628173755604No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9920.23386188004205744No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9810.23126865354965556No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGACGA8670.20439339717385463No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8620.2032146578591265No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATGTCCTCCTGT8450.19920694418905094No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8410.1982639527372684No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8200.19331324761541038No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8080.1904842732600629No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7750.18270459378285736No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7510.17704664507216242No Hit
CTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTC7360.1735104271279781No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTAAC7240.1706814527726306No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7120.16785247841728315No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7100.1673809826913919No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7060.16643799123960942No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6970.1643162604730988No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6820.16078004252891448No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA6690.15771532031062138No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAA6660.15700807672178452No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6500.15323611091465456No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTCTTCTACTG6460.15229311946287205No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6160.14522068357450338No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6150.14498493571155777No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6100.14380619639682968No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6090.14357044853388404No Hit
CTCTAAGGCCTCCCGATAGAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5960.14050572631559094No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCA5840.13767675196024348No Hit
CTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTCCT5820.13720525623435223No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5800.136733760508461No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5790.13649801264551537No Hit
ACTCCAGATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAA5790.13649801264551537No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG5740.13531927333078725No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5740.13531927333078725No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAAT5590.13178305538660293No Hit
GGCCTACATATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATG5530.1303685682089292No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA5500.12966132462009233No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5490.1294255767571467No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGTCAACTTACAAGGCC5470.12895408103125544No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5450.12848258530536422No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5420.12777534171652735No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCA5410.1275395938535817No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5370.12659660240179924No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5350.12612510667590798No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5340.12588935881296237No Hit
GTCTTACACAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5330.12565361095001673No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5310.1251821152241255No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTACTCTTCTACTGTACCTGTC5300.12494636736117987No Hit
GTGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC5220.12306038445761489No Hit
GGAACATATTGTACAAAAACTTAAGCAATGTTTTCGGAAACTGCCTGTAA5210.12282463659466927No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5050.11905267078753932No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4980.11740243574691996No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4950.11669519215808308No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4910.1157522007063006No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT4890.11528070498040936No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4890.11528070498040936No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAACCTG4850.11433771352862686No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTGTGT4770.11245173062506189No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG4720.11127299131033377No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4650.1096227562697144No Hit
ACATATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAGG4650.1096227562697144No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4640.1093870084067688No Hit
GCTTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGT4630.10915126054382317No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4600.1084440169549863No Hit
GTGTTGGGGTGAGTTGATGAACCTGGCCACCTGGGTGGGAAGTAATTTGG4580.10797252122909506No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4580.10797252122909506No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCATCAGGTCTTGCCC4540.10702952977731257No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC4530.10679378191436695No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4500.10608653832553008No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT4480.10561504259963884No Hit
GGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCG4470.1053792947366932No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA4370.10302181610723699No Hit
GTGAAAGCCTGCTTAGCTTGTATACATGCATATAAAGGCATCAAAGCAGG4360.10278606824429136No Hit
CTTTACCACAGGAACATATTGTACAAAAACTTAAGCAATGTTTTCGGAAA4350.10255032038134575No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4310.10160732892956326No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT4290.10113583320367202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)