FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375979
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC19940.5303487694791464No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA17880.47555847534037804No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT14010.37262719460395394No Hit
GTATACATGCATATAAAGGCATCAATGCAGGATAGCCACATTGTGTAAAA12810.34071051840661315No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11990.31890078967176355No Hit
CCCTAGCAGCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACCGAGT11420.3037403684780267No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11000.2925695318089574No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG10370.2758132768053535No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC10010.26623827394615124No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9500.2526736865622814No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9390.24974799124419184No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG9300.24735424052939126No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8870.2359174315586775No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8550.22740631790605326No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8440.2244806225879637No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8440.2244806225879637No Hit
GGTATAAAGGGCCTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT8290.2204910380632961No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8250.21942714885671805No Hit
GGTAAAAAGGGCCTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT8180.2175653427452065No Hit
ATGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCC8160.21703339814191752No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8150.21676742584027298No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7760.2063945060761372No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGATGTTGACATTGCTGAGAGTCCAA7590.20187297694818063No Hit
GGAACATATTGTACTAAAGCTCAAGCAATGTTTTCGGAAACTACCTGTAA7460.19841533702680206No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7120.1893722787708888No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7020.18671255575444373No Hit
TCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCCTATGGCC6990.1859146388495102No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC6850.18219102662648712No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6630.17633963599030797No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6460.17181810686235136No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6430.17102018995741783No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6410.17048824535412882No Hit
CTTTTACACAATGTGGCTATCCTGCATTGATGCCTTTATATGCATGTATA6300.16756255003603926No Hit
ATATTGTACTAAAGCTCAAGCAATGTTTTCGGAAACTACCTGTAAATAGA6150.16357296551137163No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC6120.16277504860643813No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA6010.15984935328834854No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5930.1577215748751925No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5910.1571896302719035No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5840.15532782416039193No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5800.1542639349538139No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5690.15133823963572435No Hit
ATTTACAGGTAGTTTCCGAAAACATTGCTTGAGCTTTAGTACAATATGTT5620.14947643352421278No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5560.14788059971434575No Hit
TAGCCTGTATACATGCATATAAAGGCATCAATGCAGGATAGCCACATTGT5540.14734865511105674No Hit
ACCACGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5480.1457528213011897No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACATCCGACCT5440.14468893209461167No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5410.14389101518967815No Hit
CATATAAAGGCATCAATGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5400.14362504288803365No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5370.14282712598310013No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC5350.14229518137981112No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5290.14069934756994407No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5250.13963545836336602No Hit
CTCTACCGATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGC5210.138571569156788No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5200.13830559685514351No Hit
GTACTAAAGCTCAAGCAATGTTTTCGGAAACTACCTGTAAATAGACCTAT5160.13724170764856547No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT5130.13644379074363194No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5070.13484795693376492No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5060.13458198463212043No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5050.1343160123304759No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5030.1337840677271869No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4960.13192226161567536No Hit
GTAGAGGCCCAAACGGCCCCGCGACGGGTCGTCCGCGGGATTCAGCGCCG4960.13192226161567536No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4960.13192226161567536No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC4950.13165628931403084No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4850.12899656629758577No Hit
CAAAGAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGG4830.12846462169429673No Hit
CTGTAAGAGGGCCCACGTATTGTTGACATCTATTAATAATGTCCTCCTGT4830.12846462169429673No Hit
ATCCTGCATTGATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTC4740.12607087097949618No Hit
GTCTATTTACAGGTAGTTTCCGAAAACATTGCTTGAGCTTTAGTACAATA4710.12527295407456268No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4670.12420906486798465No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA4630.12314517566140662No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4630.12314517566140662No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4600.12234725875647309No Hit
CCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT4580.1218153141531841No Hit
AGCTTGTATACATGCATATAAAGGCATCAATGCAGGATAGCCACATTGTG4570.12154934185153957No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4560.12128336954989508No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4550.12101739724825057No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGATGGGCGTTC4520.12021948034331705No Hit
TCTTACGAAAGCCCAGGATGATGGGATGGGAATACAAGTGCAGTTTCCGT4520.12021948034331705No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4510.11995350804167254No Hit
ACCAGGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4480.11915559113673901No Hit
CTTGAGTCCCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTAT4410.11729378502522748No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG4350.11569795121536043No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC4340.11543197891371593No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4320.1149000343104269No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4310.1146340620087824No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCATCAGGTCTTGCCC4270.11357017280220437No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4230.11250628359562634No Hit
GGCCTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCG4230.11250628359562634No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4220.11224031129398185No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4200.11170836669069284No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4160.11064447748411481No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4150.11037850518247029No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4090.10878267137260325No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG4070.10825072676931426No Hit
CCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCT4070.10825072676931426No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGAAATGT4040.10745280986438073No Hit
TTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAG4030.10718683756273623No Hit
CTCCCATAGGAATCTTACGAAAGCCCAGGATGATGGGATGGGAATACAAG4010.1066548929594472No Hit
GGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAG3970.10559100375286917No Hit
ATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCT3940.10479308684793566No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA3900.10372919764135764No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3880.1031972530380686No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA3840.10213336383149059No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC3820.10160141922820158No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT3820.10160141922820158No Hit
CTTTACCACAGGAACATATTGTACTAAAGCTCAAGCAATGTTTTCGGAAA3820.10160141922820158No Hit
GGGCCTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATA3770.10027155771997905No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)