FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461534
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG24650.5340884961887965No Hit
TTGTTCACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGG12860.2786360268149259No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12840.2782026892926631No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12530.27148595769759104No Hit
ATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAA11750.2545857943293452No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT11580.2509024253901121No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11450.24808573149540444No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG11270.24418569379504002No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA10910.23638561839431113No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG10200.22100213635398475No Hit
GTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAG10190.2207854675928534No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9790.2121187171475991No Hit
GTTTTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT9740.2110353733419423No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9500.20583532307478972No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT9430.20431864174687023No Hit
CTAACAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATGTA9420.20410197298573887No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9280.20106861032989984No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8740.18936849722880655No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAGGTAAGA8610.18655180333409888No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8230.1783183904111073No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTCCTAAC8180.1772350466054505No Hit
GTTCACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAG8100.17550169651639966No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8070.1748516902330056No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTATAAGGCC8050.17441835271074288No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7990.17311834014395472No Hit
GATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTA7910.17138499005490387No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATA7810.1692183024435903No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA7210.15621817677570884No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA7130.15448482668665797No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7110.15405148916439523No Hit
CTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATGTA7040.15253480783647574No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6800.14733475756932315No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCCTTTC6770.1466847512859291No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6740.14603474500253502No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6720.1456014074802723No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6670.14451806367461553No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6660.14430139491348415No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGCACCTTTACCCCGTTGCC6580.1425680448244333No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6530.14148470101877653No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6480.14040135721311972No Hit
CATTTAAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT6410.13888467588520023No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6390.1384513383629375No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6160.1334679568569163No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6150.13325128809578493No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6110.13238461305125948No Hit
ATTGACAACAGTGCCCTCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6110.13238461305125948No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5880.12740123154523827No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5880.12740123154523827No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5870.1271845627841069No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT5860.12696789402297556No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5860.12696789402297556No Hit
CTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5850.12675122526184418No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5840.12653455650071285No Hit
ATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5680.12306785632261112No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5650.12241785003921704No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5630.12198451251695433No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5590.1211178374724289No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5500.11916781862224668No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5490.11895114986111534No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5470.1185178123388526No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5370.11635112472753904No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5340.11570111844414496No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT5290.11461777463848818No Hit
TTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACAC5240.11353443083283138No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATATGTT5210.1128844245494373No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGG5180.11223441826604323No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5150.11158441198264915No Hit
GATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTA5090.11028439941586103No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5060.10963439313246695No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC5040.10920105561020423No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAAT4990.10811771180454745No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4950.10725103676002201No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4940.10703436799889066No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4910.10638436171549659No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4900.10616769295436522No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4880.10573435543210251No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTTTCACCTGCCGGACCGTGTG4870.10551768667097115No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4830.10465101162644573No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAT4800.10400100534305166No Hit
AGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA4740.10270099277626352No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4680.10140098020947537No Hit
GTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT4650.10075097392608129No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAAGAACATATTGTACAAAAA4650.10075097392608129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)