FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350794
Sequences flagged as poor quality0
Sequence length35-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCAGG20650.5886645723701089No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14300.40764665302143144No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11490.3275426603647725No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA10450.297895631054123No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10380.29590015792744456No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9860.2810766432721198No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTATGTTCA7940.2263436660832283No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7360.2098097458907507No Hit
GATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT6790.19356089328779852No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6470.18443873042298328No Hit
GTTTTGAAGTAATGATTAACTGTATGTTCAGGATAATAAGGTTTAATGCC6340.1807328517591521No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5850.16676453987240375No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5800.16533920192477636No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5570.15878264736569042No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGTA5510.15707224182853755No Hit
ATTTCAATCTCCTTTTTTCATTAACTGTAAGAGGGCCCACATATTGTTGA5250.14966048450087516No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAACATTCATTTACAAG5100.145384470657993No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAAGCCTTGTAAGTTGGCGA4720.13455190225602492No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTGAAATTGATTATGCCTGCTAGG4470.127425212517888No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA4440.12657000974931157No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4430.12628494215978608No Hit
GATTAAAATTGATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT4060.11573744134734346No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4010.11431210339971608No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA3990.11374196822066512No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT3660.1043347377663244No Hit
CCCTCATAAAACCAAGCGTTGGGGCTACTCCCTTAATTTCATGGGATATG3660.1043347377663244No Hit
CAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG3520.10034379151296771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)