FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259218
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCCTAT8670.3344675138300581No Hit
CCTCCTGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6550.2526830698485445No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6160.23763781836137923No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6030.23262273453232415No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAAACC5940.2291507534199014No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5660.21834903440347506No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5520.2129481748952619No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5320.20523266131210025No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG5310.20484688563294218No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5070.19558826933314816No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5030.19404516661651583No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4660.17977146648766676No Hit
GTACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT4540.17514215833776975No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGTTTTGTAAGTTGGCGA4130.15932535549228835No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4030.15546759870070753No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA3840.14813786079670394No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA3820.14736630943838777No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3740.14428010400512312No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC3720.14350855264680695No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3680.1419654499301746No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGCGTAAAC3660.14119389857185843No Hit
CTATATAAGAGAGAAACTACACGCAGTGCCTCATTCTGTGGGTCACCATA3570.13772191745943568No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC3530.13617881474280336No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA3430.13232105795122254No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3350.12923485251795785No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTGTTCG3330.1284633011596417No Hit
GCCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTG3320.12807752548048362No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3280.12653442276385127No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3220.12421976868890279No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3190.12306244165142853No Hit
GTTTTTGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACA3090.11920468485984768No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG3080.11881890918068962No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA3080.11881890918068962No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC2960.1141896010307926No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC2930.11303227399331837No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT2900.11187494695584413No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG2900.11187494695584413No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2890.11148917127668603No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAT2840.10956029288089561No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG2840.10956029288089561No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT2790.1076314144851052No Hit
GAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTTCCGATA2790.1076314144851052No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT2750.10608831176847286No Hit
GGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT2740.10570253608931479No Hit
GTTTAGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA2730.1053167604101567No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA2720.10493098473099863No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC2710.10454520905184053No Hit
CTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT2700.10415943337268245No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2670.10300210633520819No Hit
ATATAAGAGAGAAACTACACGCAGTGCCTCATTCTGTGGGTCACCATATT2670.10300210633520819No Hit
GTATCGGGAAGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG2650.10223055497689203No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT2620.10107322793941778No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTATCTGCCAAGTGTTTGCTG2600.10030167658110162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)