FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259218
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA7570.292032189122669No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCCTAT7170.2766011619563456No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6850.264256340223287No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6260.24149557515296005No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAAACC5770.222592566874214No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5670.21873481008263315No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5650.217963258724317No Hit
GTACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT5440.20986196946199726No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5260.20291800723715175No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4830.18632965303335416No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA4670.18015724216682483No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4550.17552793401692784No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGCGTAAAC4130.15932535549228835No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4100.1581680284548141No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4040.1558533743798656No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG3820.14736630943838777No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA3800.14659475808007158No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGTTTTGTAAGTTGGCGA3770.14543743104259735No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3680.1419654499301746No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3650.14080812289270034No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3650.14080812289270034No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC3580.13810769313859378No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA3570.13772191745943568No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTGTTCG3560.13733614178027762No Hit
CCTCCTTTCCATGGCTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGG3500.1350214877053291No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3490.13463571202617103No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3460.13347838498869677No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC3430.13232105795122254No Hit
GTTTTTGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACA3400.13116373091374828No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC3390.13077795523459018No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT3300.12730597412216743No Hit
CTATATAAGAGAGAAACTACACGCAGTGCCTCATTCTGTGGGTCACCATA3260.1257628714055351No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTATCTGCCAAGTGTTTGCTG3150.12151933893479619No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC3130.12074778757648003No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTACAATA3090.11920468485984768No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT3070.11843313350153152No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3020.11650425510574111No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA3020.11650425510574111No Hit
GTATCGGGAAGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG2980.11496115238910878No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG2960.1141896010307926No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT2940.11341804967247644No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2910.1122607226350022No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG2850.1099460685600537No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC2780.10724563880594712No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC2740.10570253608931479No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2710.10454520905184053No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA2680.10338788201436629No Hit
GTTTAGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA2650.10223055497689203No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAT2640.10184477929773396No Hit
GTTCAAATGTATGCCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA2640.10184477929773396No Hit
GAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTTCCGATA2630.10145900361857588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)