FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469026
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34290.7310895344820969No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTTACTGTAAATGAAAAGAG18590.3963532938472494No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT14910.31789282470481384No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT13740.29294751250463724No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC11340.24177764132478793No Hit
ATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCCATGAA11300.24092481013845715No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC10650.2270663033605813No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT10530.2245078098015888No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA10400.22173610844601366No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG10330.22024365386993472No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10220.21789836810752494No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG10180.21704553692119413No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10120.2157662901416979No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA9820.20937005624421676No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT9420.20084174438090852No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA9380.1999889131945777No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC9280.19785683522875064No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT9210.1963643806526717No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT9140.19487192607659276No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG9040.1927398481107657No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT8950.19082097794152136No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8870.18911531556885972No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG8710.18570399082353642No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTTACTGTAAATGAAAAG8520.18165304268846502No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT8480.1808002115021342No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA8020.17099265285932977No Hit
TTCAAGTACCGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA7610.16225113319943885No Hit
CTCTTACTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGA7570.161398302013108No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA7530.1605454708267772No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC7510.16011905523361178No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC7460.15905301625069826No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA7400.15777376947120203No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC7360.1569209382848712No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT7290.15542848370879225No Hit
CTCCGAAAGAGACCAAATATTCAAGTACCGTCTCTCTTCCAAAAGTAAGG7280.15521527591220954No Hit
GTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCC7270.15500206811562686No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG7060.15052470438739002No Hit
GGACATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTTACTGTAAATG6760.14412847048990887No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA6710.14306243150699535No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG6670.14220960032066454No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6520.13901148337192396No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA6380.13602657421976605No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT6380.13602657421976605No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6350.13538695083001795No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6210.13240204167786007No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6170.13154921049152926No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA6150.13112279489836384No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTT5960.12707184676329245No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5770.12302089862822105No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC5750.12259448303505562No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA5690.1213152362555594No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5660.12067561286581129No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT5620.11982278167948046No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTTCCCCAACTTC5480.11683787252732258No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG5450.11619824913757446No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT5440.11598504134099176No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC5300.11300013218883387No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT5190.11065484642642412No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC5140.1095888074435106No Hit
CAGTAAGAGGGCCCACAAATTGTTGACACCTATTAATAATGTCCTCTTGT5040.10745672947768353No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC5010.10681710608793542No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT4930.10511144371527377No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC4870.10383219693577755No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT4860.10361898913919484No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4830.10297936574944673No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4820.10276615795286402No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA4750.10127370337678508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)