FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204335
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA25301.2381628208579047No Hit
GTTCAGGAACAGTGAGCCCTGCTCAGAATACTGCCTCTGCCATATCGTCA17590.8608412655687964No Hit
AGTTCAGGAACAGTGAGCCCTGCTCAGAATACTGCCTCTGCCATATCGTC16420.8035823525093596No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG16290.7972202510583112No Hit
GTACAGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT14990.7335992365478259No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC11060.541268015758436No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA10230.5006484449555876No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATTCTTTGACATACT9420.46100765899136226No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC9290.4546455575403137No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATTACATATCCCATGAA7880.3856412264174028No Hit
ATACTATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA7540.36900188416081436No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGCAGAACCAA7440.36410795996770007No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7320.358235250935963No Hit
GTTTAGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT7220.35334132674284874No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6760.33082927545452323No Hit
CTCACCAACTTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC6480.3171262877138033No Hit
ACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACG6000.29363545158685495No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6000.29363545158685495No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5630.27552793207233217No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5590.2735703623950865No Hit
GGGTAAAGGTTCAGATACTGGTTACGTAAAAAGGCCTTATAAGTTGGCGA5320.260356767073678No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATTCT5160.2525264883646952No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGGTTACGT5130.2510583111067609No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA5120.2505689186874495No Hit
CACTCACCAACTTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTG5030.24616438691364673No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4920.24078107030122103No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATATGGTATTGGGGGC4900.2398022854625982No Hit
GACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTGTGTCT4770.23344018401154965No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGCAGAACCAAC4740.23197200675361537No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAAT4610.22560990530256686No Hit
GGTTACGTAAAAAGGCCTTATAAGTTGGCGAGAAGGTAAAAGCCTGTTTA4600.22512051288325544No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA4590.22463112046394404No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT4580.2241417280446326No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4580.2241417280446326No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4470.2187584114322069No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4470.2187584114322069No Hit
GGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGG4430.2168008417549612No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4370.21386448723909268No Hit
CATTTAAACCCTCACAAAACAAAAAGGTGGGGATATTCCCTTAATTTCAT4350.2128857024004698No Hit
GAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGACCCTG4350.2128857024004698No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4270.2089705630459784No Hit
GTACAGACTTGGCCCCCAATACCATATCATCCATATAACTGAAAGCCAGA4080.19967210707906133No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3960.19379939804732427No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3950.19331000562801282No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC3950.19331000562801282No Hit
GGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCAAACCCCAA3890.19037365111214427No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA3880.18988425869283285No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCACCTTTTTGTTTTG3770.18450094208040718No Hit
CTCTACTTCCAGGATCATCAACCACCAGCACCGGACCATGCAAAACCTGC3670.17960701788729294No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGT3660.1791176254679815No Hit
GCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGACCCTGTACAGAA3580.1752024861134901No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT3520.17226613159762155No Hit
CACTTGGCACAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGAT3510.17177673917831013No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA3450.16884038466244158No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA3450.16884038466244158No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA3450.16884038466244158No Hit
TGATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATG3430.16786159982381874No Hit
TACCTTGATAGTCCAGCAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3420.1673722074045073No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC3420.1673722074045073No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA3410.1668828149851959No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG3400.16639342256588446No Hit
CCCAATTACATATCCCATGAAATTAAGGGAATATCCCCACCTTTTTGTTT3370.1649252453079502No Hit
GCACCGGATCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG3320.16247828321139304No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT3290.16101010595345877No Hit
GCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGC3260.1595419286955245No Hit
CTTCAAAAAGTTGCATGGTGCTGGTGAACAGACCAATTTATGCCTACAGC3200.15660557417965595No Hit
ACCCTGTACAGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG3170.15513739692172168No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATG3100.1517116499865417No Hit
GGTAAAAGCCTGTTTAGCTTGTATACATGCATATAAAGGCATTAAAGCAG3040.14877529547067317No Hit
GTCTAGACTTGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG3030.14828590305136172No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATT3020.14779651063205032No Hit
TAAACGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCATATCATC3020.14779651063205032No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA3020.14779651063205032No Hit
GGTTCAGATACTGGTTACGTAAAAAGGCCTTATAAGTTGGCGAGAAGGTA2950.14437076369687032No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGT2930.14339197885824748No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCA2910.14241319401962463No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC2890.14143440918100178No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCATATC2860.1399662319230675No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCAGGCC2860.1399662319230675No Hit
CAGTTCAGGAACAGTGAGCCCTGCTCAGAATACTGCCTCTGCCATATCGT2770.1355617001492647No Hit
ATCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC2770.1355617001492647No Hit
CATGTATACAAGCTAAACAGGCTTTTACCTTCTCGCCAACTTATAAGGCC2740.13409352289133042No Hit
ATGTAATTGGGAGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA2740.13409352289133042No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2730.133604130472019No Hit
CAGTGAGCCCTGCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCG2670.13066777595615045No Hit
GCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCCTATGGCCAAG2670.13066777595615045No Hit
GAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGTGTTAC2630.12871020627890475No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA2620.1282208138595933No Hit
CCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTGCTGGACT2600.12724202902097045No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC2590.12675263660165906No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATAATTAACCT2560.12528445934372476No Hit
ATCTTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT2550.12479506692441335No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTGCATCCT2540.12430567450510191No Hit
TCCACATTGTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATTCTTT2520.12332688966647906No Hit
CCCCAATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAG2520.12332688966647906No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC2520.12332688966647906No Hit
ATACAATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA2500.12234810482785621No Hit
GTACAATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATTACATATCCC2490.1218587124085448No Hit
ATATTGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC2480.12136931998923337No Hit
AGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTATTT2480.12136931998923337No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT2470.12087992756992195No Hit
CTCACTAACTTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC2470.12087992756992195No Hit
CCTTTTTGTTTTGTGAGGGTTTAAATGTATACCCAAAGACAAAAGAAAAT2440.11941175031198767No Hit
TGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATG2430.11892235789267623No Hit
ATGTTGTACAGACTTGGCCCCCAATACCATATCATCCATATAACTGAAAG2430.11892235789267623No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCCGGTCTG2420.11843296547336482No Hit
CTAACCAACTTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC2410.11794357305405338No Hit
TGGTTAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACACGG2390.11696478821543053No Hit
GACTTGGCCCCCAATACCATATCATCCATATAACTGAAAGCCAGACAGTG2380.11647539579611912No Hit
GTACAGAACATGGAGAACATCGCATCAGGACACCTAGGACCCCTGCTCGT2360.11549661095749628No Hit
GTTAGTGAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACAC2350.11500721853818484No Hit
CCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGCCTGT2330.11402843369956199No Hit
GTCCAGCAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA2330.11402843369956199No Hit
CACCAGCACCATGCAACTTTTTGAAGAGCTCAAGCTTTCCGGAAAGCTTG2320.11353904128025058No Hit
TCTACTTCCAGGATCATCAACCACCAGCACCGGACCATGCAAAACCTGCA2290.1120708640223163No Hit
CCACAAGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAA2270.11109207918369345No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAGTTTCCGTCCGTAGGTTT2270.11109207918369345No Hit
ACTTATCGGGACTGACAATTCTGTCGTGCTCTCCCGCAAGTATACATCAT2260.11060268676438202No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG2230.10913450950644774No Hit
GTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTGTGTCTTGGCC2220.10864511708713633No Hit
GCCATATCGTCAACCTTCTCGAAGACTGGGGACCCTGTACAGAACATGGA2220.10864511708713633No Hit
AAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTA2210.10815572466782489No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA2190.10717693982920204No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGG2180.10668754740989063No Hit
GTTTTGTACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTC2150.10521937015195634No Hit
CATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCAAACCCCAAAA2150.10521937015195634No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC2130.1042405853133335No Hit
ATATAGTCTTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC2120.10375119289402206No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAAGAGAAA2110.10326180047471065No Hit
TAGCAGCAGGATGCAGAGGAAGATGATAAAACGCCGCAGACACATCCAGC2100.10277240805539921No Hit
CAATCTAAACAGGCTTTTACCTTCTCGCCAACTTATAAGGCCTTTTTACG2090.1022830156360878No Hit
CTGTTTACTTAAAAAGGCCTTATAAGTTGGCGAGAAGGTAAAAGCCTGTT2060.10081483837815351No Hit
GTTCAGATACTGGTTACGTAAAAAGGCCTTATAAGTTGGCGAGAAGGTAA2050.1003254459588421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)