FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461228
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35310.7655649700365111No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG19560.4240852680236239No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT18870.4091252048878212No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT14950.3241347012757248No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGGAAGGAAGGAGTTT14410.31242682577814007No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC12170.26386082371408504No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT11200.24283001032027546No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG11090.2404450727189156No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC10910.23654244755305404No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10720.2324230098779779No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT10390.2252681970738984No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA9890.2144275716131718No Hit
TCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGG9830.21312669655788463No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT9830.21312669655788463No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT9820.2129098840486701No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG9600.20814000884595038No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC8960.19426400825622034No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTCCCTAAG8790.1905781955995733No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG8660.1877596329797844No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT8590.1862419454152827No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC8400.18212250774020658No Hit
GCCTCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCC7760.16824650715047657No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA7720.16737925711361842No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA7670.16629519456754577No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7630.16542794453068765No Hit
ATATGCTCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA7490.1623925694016842No Hit
GGAGCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA7410.16065806932796795No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT7400.1604412568187534No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG7380.16000763180032435No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA7250.15718906918053543No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7220.15653863165289186No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA7180.15567138161603372No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7170.15545456910681918No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7140.1548041315791756No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA7030.15241919397781575No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG6490.14071131848023105No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC6490.14071131848023105No Hit
GCCTAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGTCCTTAA6450.1398440684433729No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT6400.13876000589730025No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA6370.13810956836965665No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC6350.1376759433512276No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT6320.137025505823584No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT6220.1348573807314387No Hit
CCTTTATACCGCTGTTACCAATTTTCTTGTGTCTCTGGGTATACATTTAA6110.13247244313007883No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6060.13138838058400618No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGCTCCTGTGGCAATGTT6050.13117156807479163No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6000.130087505528719No Hit
GTACAATATGCTCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC5980.12965388051028992No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG5750.1246671927983557No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC5720.12401675527071211No Hit
ATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAG5580.12098138014170864No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA5570.12076456763249412No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5550.12033094261406506No Hit
ATTCGTTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT5380.11664512995741803No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA5270.11426019235605818No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG5220.11317612980998551No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC5190.11252569228234192No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTCC5180.1123088797731274No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTCCCTAAGATTCATTTACAAG5150.1116584422454838No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC5060.10970712966255301No Hit
TCTCCTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTT5040.10927350464412394No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT4790.10385319191376066No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA4790.10385319191376066No Hit
CTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGATTCTAT4780.10363637940454613No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA4730.10255231685847346No Hit
GTGTTTGATCTTTTGTACAATATGCTCCTGTGGCAATGTTCCCCAACTTC4720.10233550434925894No Hit
ATTTACAAGAGGACATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTC4720.10233550434925894No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG4660.10103462929397175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)