FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461228
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35240.7640472824720095No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG19230.41693045521954436No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT15950.345815952197178No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGGAAGGAAGGAGTTT13440.2913960123843305No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT12860.2788208868498877No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG11040.23936101017284295No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC10770.2335070724240506No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT10440.22635225961997102No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10380.22505138456468385No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT10190.22093194688960774No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC10040.21767975925138977No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT9570.20748957131830678No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT9400.20380375866165973No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG9320.2020692585879435No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG9220.19990113349579816No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA9170.19881707094972553No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT9080.19686575836679474No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTCCCTAAG8690.18841007050742797No Hit
TCTTTTGTCTCTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGG8550.18537469537842455No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC8130.17626856999141421No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7900.17128188227948No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT7880.17084825726105093No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC7810.1693305696965492No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA7630.16542794453068765No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7590.1645606944938295No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA7520.1630430069293278No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA7270.1576226941989645No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT7270.1576226941989645No Hit
ATATGCTCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA7160.15523775659760466No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG7100.15393688154231747No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7010.1519855689593867No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA6930.1502510688856704No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA6870.14895019383038324No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6830.1480829437935251No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6710.14548119368295073No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG6680.14483075615530713No Hit
GCCTCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCC6620.14352988110001996No Hit
GGAGCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA6430.13941044342494385No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG6380.13832638087887117No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6380.13832638087887117No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGCTCCTGTGGCAATGTT6300.13659188080515494No Hit
CCTTTATACCGCTGTTACCAATTTTCTTGTGTCTCTGGGTATACATTTAA6250.13550781825908226No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT6230.1350741932406532No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG6230.1350741932406532No Hit
GTACAATATGCTCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC6000.130087505528719No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT5690.1233663177430685No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA5620.12184863017856679No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC5600.12141500516013773No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTCCCTAAGATTCATTTACAAG5550.12033094261406506No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5510.11946369257720693No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC5490.11903006755877787No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA5490.11903006755877787No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5410.11729556748506162No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTCC5370.11642831744820348No Hit
ATTCGTTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT5200.11274250479155645No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC5180.1123088797731274No Hit
GTGTTTGATCTTTTGTACAATATGCTCCTGTGGCAATGTTCCCCAACTTC5180.1123088797731274No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA5140.11144162973626927No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT5060.10970712966255301No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC5030.10905669213490941No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA4970.10775581707962222No Hit
TCTCCTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTT4970.10775581707962222No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG4930.1068885670427641No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG4900.10623812951512049No Hit
GCCTAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGTCCTTAA4870.10558769198747692No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTCCCT4850.10515406696904786No Hit
ATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAG4810.10428681693218972No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG4680.10146825431240081No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT4630.10038419176632814No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)