FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445789
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC47691.0697886219713812No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA19170.43002406968319096No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAAAGTCCAA17420.3907678296234317No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16930.37977608240669913No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC15560.34904405447420195No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC15330.3438846629234907No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC12410.27838282236663536No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCTGTAATGTCAACGACCGACCT12130.2721018239570739No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11220.25168857912599907No Hit
CCCCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11220.25168857912599907No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATACGTTGACAGACT9790.21961062296288153No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG9780.21938630159111147No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC9280.20817023300260884No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA9160.20547837654136822No Hit
CTATGCATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC9110.20435676968251795No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCTTTAATCTAATCTCCTCC8680.1947109506964057No Hit
ATATTAGTGTTGACATAACTAACTACTAGGTCCCTAGATGCTGGATCTTC8190.1837192034796731No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8180.18349488210790305No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8180.18349488210790305No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT8070.18102734701843248No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT7980.17900845467250204No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTTTTGTGGTTTCACATTTCTT7940.1781111691854218No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7280.16330595864859834No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7240.16240867316151814No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCGTCTGCCGGACCGTGTG7090.15904385258496734No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7010.15724928161080692No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG6870.15410878240602616No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT6840.153435818290716No Hit
CCCTTATCTAATGGTAAATATTTGGTAACATTTGGGTAAAACCTAGCAGG6790.15231421143186574No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA6730.15096828320124545No Hit
GTTTAATACCCTTATCTAATGGTAAATATTTGGTAACATTTGGGTAAAAC6700.15029531908593527No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6620.14850074811177486No Hit
CTCTTGGACTTTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA6560.14715481988115453No Hit
GATCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC6520.14625753439407432No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6510.1460332130223043No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG6440.1444629634199139No Hit
TCCTAGTACAAAGATCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT6410.14378999930460373No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6350.14244407107398344No Hit
GGATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGTTCC6290.1410981428433631No Hit
CTTCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGA6260.14042517872805296No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG6220.13952789324097276No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6170.1384062863821225No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6160.13818196501035243No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG6150.13795764363858237No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6140.1377333222668123No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC6120.13728467952327222No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC6100.1368360367797321No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCCGCATCTACCAATCGCCAGTC6080.13638739403619202No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCTTTAATCTAATCT6010.13481714443380166No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5930.13302257345964122No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5900.13234960934433104No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5900.13234960934433104No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5870.1316766452290209No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5870.1316766452290209No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA5860.13145232385725084No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCCCCAGC5840.13100368111371075No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5650.12674157505007974No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5510.12360107584529902No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAAAGTCCAAGAGTCCTCTTATGT5460.12247946898644875No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5400.12113354075582843No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5360.12023625526874822No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATTCAATCTCGGC5260.1179930415510477No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGTTCCC5230.11732007743573754No Hit
CTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC5190.11642279194865732No Hit
GTACAAAGATCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA5190.11642279194865732No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT5130.115076863718037No Hit
CTCCCCGTCTGTGCCTTCTCGTCTGCCGGACCGTGTGCACTTCGCTTCAC5130.115076863718037No Hit
GGTTTAATACCCTTATCTAATGGTAAATATTTGGTAACATTTGGGTAAAA5120.11485254234626695No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCT5120.11485254234626695No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA5040.11305797137210653No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4990.11193636451325628No Hit
GTCGTTGACATTACAGAAAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4970.11148772176971618No Hit
TCTAGATACCGCCTCAGCTCTCTATCGGGAAGCCTTAGAGTCTCCTGAGC4950.11103907902617607No Hit
ATCTGAACCTTTACCCCGTTGTTCGGCAACGGCCAGGTCTGTGCCAAGTG4860.10902018668024559No Hit
TCTAGGGACCTAGTAGTTAGTTATGTCAACACTAATATGGGCCTAAAGTT4820.10812290119316538No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4760.10677697296254507No Hit
CCTCTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCGTCTGCCGGACCGT4740.10632833021900497No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4740.10632833021900497No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACTAATTTTCTT4700.10543104473192476No Hit
GCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAGACTAC4620.10363647375776432No Hit
ACATAAGAGGACTCTTGGACTTTCTGTAATGTCAACGACCGACCTTGAGG4550.10206622415537396No Hit
GGGTAAAGGTTCAGATATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGA4530.10161758141183386No Hit
CTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTC4500.1009446172965237No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4500.1009446172965237No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT4480.10049597455298359No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA4480.10049597455298359No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG4460.10004733180944349No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCTTAT4460.10004733180944349No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)