FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325898
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14580.4473792413577254No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA13350.4096373712020325No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12760.3915335473062124No Hit
TAACCACACTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG12490.3832487465403286No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA12220.37496394577444475No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10160.3117539843754794No Hit
GTGTGGTTATCCTGCTTTAATGCCCTTATATGCATGTATTCAGTCTAAGC10130.3108334509570479No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9680.2970254496805749No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAACC8650.2654204689810923No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8160.2503850898133772No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7320.22461015409729426No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7220.22154170936918913No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6830.20957477492957918No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6770.2077337080927161No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6420.19699415154434824No Hit
ACATCGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT6040.1853340615775488No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT5840.17919717212133857No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5620.17244659371950735No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5610.1721397492466968No Hit
GTATAATGTGATCTTGAGGCAATGATCCATAACATCCAATGACATAACCC5510.1690713045185917No Hit
TCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA5460.16753708215453914No Hit
GGATACTGGATCTTCCAAATTTCCACCCACCCAGGTAGCTAGAGTCATTA5420.1663097042632971No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC5360.16446863742643406No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT5230.16047965927989738No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5170.15863859244303433No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAATACT4980.15280854745963462No Hit
GTGTAAACAATACCAGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4790.1469785024762349No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTATTCAAGCCTCCAAGCTGTGC4710.1445237466937508No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA4690.1439100577481298No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT4670.14329636880250876No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4640.14237583538407722No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4540.1393073906559721No Hit
ATACTGGATGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAG4540.1393073906559721No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCTCTCAGGCA4510.1383868572375406No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAATACTGGATGGGCGTTC4430.1359321014550565No Hit
CTTTTACACAGTGTGGTTATCCTGCTTTAATGCCCTTATATGCATGTATT4410.13531841250943547No Hit
GTATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4370.13409103461819344No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4290.13163627883570933No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4230.12979521199884628No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA4220.12948836752603576No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4210.12918152305322522No Hit
AATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCT4160.12764730068917268No Hit
GTATGGTGAGGTGAACAATGCTCAGGAGACTCTAAGGCTTCCCGATACAG4150.12734045621636217No Hit
GAATACATGCATATAAGGGCATTAAAGCAGGATAACCACACTGTGTAAAA4060.12457885596106757No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4010.12304463359701502No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT3990.122430944651394No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT3950.12120356676015194No Hit
GGGTAAAGGTTCTGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3930.12058987781453093No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA3880.11905565545047836No Hit
GACATCGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTT3860.11844196650485735No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT3860.11844196650485735No Hit
ACATTATACAGAAAATCAAAGAATGTTTTCGAAAACTTCCTGTTAACAGG3830.11752143308642582No Hit
ACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGAGCT3790.11629405519518377No Hit
GGGAAATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT3720.11414614388551018No Hit
ACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCG3650.1119982325758366No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT3640.1116913881030261No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA3610.11077085468459456No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3600.11046401021178405No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3590.11015716573897355No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC3580.10985032126616304No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT3570.10954347679335254No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC3530.10831609890211047No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG3510.10770240995648946No Hit
ATGCAAGACCTTGGGCAATACTGGATGGGCGTTCACGGTGGTCTCCATGC3490.10708872101086843No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3490.10708872101086843No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTCTGT3470.10647503206524742No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC3440.10555449864681588No Hit
CATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3410.10463396522838433No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3360.10309974286433178No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA3340.10248605391871075No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCAAGC3330.10217920944590025No Hit
CATATAAGGGCATTAAAGCAGGATAACCACACTGTGTAAAAGGGGCAGCA3320.10187236497308974No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT3310.10156552050027924No Hit
TCCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTAATGTGGGCC3310.10156552050027924No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT3270.10033814260903717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)