FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325898
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14560.4467655524121044No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA13470.41331950487575864No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12430.3814076797034655No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA11500.35287114373208794No Hit
TAACCACACTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG10170.31206082884828995No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9520.2921159381156067No Hit
GTGTGGTTATCCTGCTTTAATGCCCTTATATGCATGTATTCAGTCTAAGC9150.2807626926216178No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC8920.27370526974697607No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8490.26051095741612407No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8340.2559082903239664No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAACC8220.2522261566502403No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7080.217245886749842No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA6890.21141584176644226No Hit
ACATCGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT6630.20343788547336894No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5990.18379983921349624No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5930.18195877237663316No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5840.17919717212133857No Hit
GTATAATGTGATCTTGAGGCAATGATCCATAACATCCAATGACATAACCC5830.17889032764852808No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5790.17766294975728603No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5400.16569601531767608No Hit
GGATACTGGATCTTCCAAATTTCCACCCACCCAGGTAGCTAGAGTCATTA5330.16354810400800252No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTATTCAAGCCTCCAAGCTGTGC5310.1629344150623815No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT5200.15955912586146584No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT5180.15894543691584484No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT5130.15741121455179227No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5000.15342223640525562No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4910.15066063614996103No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAATACT4690.1439100577481298No Hit
GTGTAAACAATACCAGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4670.14329636880250876No Hit
AATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCT4640.14237583538407722No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4530.1390005461831616No Hit
GACATCGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTT4520.13869370171035109No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC4490.13777316829191957No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4400.13501156803662495No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCTCTCAGGCA4380.13439787909100392No Hit
CTTTTACACAGTGTGGTTATCCTGCTTTAATGCCCTTATATGCATGTATT4290.13163627883570933No Hit
GTATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4220.12948836752603576No Hit
GGGTAAAGGTTCTGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4200.12887467858041474No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4200.12887467858041474No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG4180.1282609896347937No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4150.12734045621636217No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4140.12703361174355166No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAA4090.1254993893794991No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT4070.12488570043387807No Hit
GAATACATGCATATAAGGGCATTAAAGCAGGATAACCACACTGTGTAAAA4070.12488570043387807No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAATACTGGATGGGCGTTC4000.1227377891242045No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA3930.12058987781453093No Hit
CTACTATTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGAC3910.1199761888689099No Hit
ATACTGGATGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAG3830.11752143308642582No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC3830.11752143308642582No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC3820.1172145886136153No Hit
TCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA3810.11690774414080478No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT3770.11568036624956274No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT3760.11537352177675222No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA3740.11475983283113121No Hit
GTATGGTGAGGTGAACAATGCTCAGGAGACTCTAAGGCTTCCCGATACAG3690.11322561046707864No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3640.1116913881030261No Hit
GGATAACCACACTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3590.11015716573897355No Hit
ACACTAATGTGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCT3580.10985032126616304No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT3580.10985032126616304No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT3560.10923663232054201No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT3550.1089297878477315No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT3540.108622943374921No Hit
TCCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTAATGTGGGCC3520.10800925442929997No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC3510.10770240995648946No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC3430.10524765417400535No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCGAAAACATTCTTTGATTTTCTGT3420.10494080970119485No Hit
CATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3420.10494080970119485No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA3410.10463396522838433No Hit
ACATTATACAGAAAATCAAAGAATGTTTTCGAAAACTTCCTGTTAACAGG3350.10279289839152128No Hit
CTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA3350.10279289839152128No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG3340.10248605391871075No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC3320.10187236497308974No Hit
ACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGAGCT3310.10156552050027924No Hit
ATCCAGTATCCAGGGACCTAGTAGTCAGTTATGTCAACACTAATGTGGGC3300.10125867602746871No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCAAGC3290.1009518315546582No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT3290.1009518315546582No Hit
CATCGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTT3290.1009518315546582No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3280.1006449870818477No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3270.10033814260903717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)