FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381131
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTTTCCATGC22040.5782788594997521No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC21010.5512540307663244No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATCAACTAGA16880.44289233885461954No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14170.3717881778181255No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13820.36260498358832005No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC11370.2983226239796815No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT11210.2941245923317704No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC9910.2600155851924929No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC9500.2492581290947207No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA9100.23876304997494302No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT8680.2277432168991764No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8410.22065903849332646No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTAT8410.22065903849332646No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8340.21882239964736536No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7960.2088520744835765No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATCAACTAGATGTTCTGGAT7730.20281740398970433No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT7560.1983569953637988No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT7460.19573322558385434No Hit
CTCTTATATAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTTTC7170.18812429322201552No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT6920.18156486877215447No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6810.17867872201421559No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6780.17789159108023225No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCATCAAACTCTGCAAG6660.17474306734429895No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGATTTGGTGGGCGTTC6610.17343118245432673No Hit
AATATACATCGTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG6550.17185692058636007No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA6530.17133216663037118No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCA6520.17106978965237676No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA6430.16870839685042674No Hit
CACTAATATGGGCCTAAAGTTTAGACAACTCTTGTGGTTTCACATTTCTT6290.16503511915850455No Hit
GTTATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCAAGC6200.16267372635655458No Hit
ATGTATATTTGCGGGAGAGGACAACAGAGTTATCAGTCCCGATAATGTTT6190.16241134937856014No Hit
ATATTAGTGTTGACATAACTGACTATTAGGTCTCTAGATGCTTGATCTTC6000.1574261867966657No Hit
GTATAAAGGGCCTCAAGATGCGGTACAGACTTGGCCCCCAATACCACATC5980.1569014328406768No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC5950.1561143019066935No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5910.15506479399471573No Hit
TACCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5840.15322815514875462No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATAA5730.15034200839081577No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT5730.15034200839081577No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5580.1464063537208991No Hit
GCCATAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC5520.14483209185293244No Hit
CTATAAGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTC5510.14456971487493803No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5400.14168356811699914No Hit
CCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5290.13879742135906026No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT5120.13433701273315474No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5100.13381225877716585No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA5070.13302512784318252No Hit
CATTCTCGGGACTGATAACTCTGTTGTCCTCTCCCGCAAATATACATCGT5060.1327627508651881No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA4990.130926112019227No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTG4880.1280399652612881No Hit
GTACCGCATCTTGAGGCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC4870.12777758828329366No Hit
GCCTAGCAGCCATGGGAACGATGTATATTTGCGGGAGAGGACAACAGAGT4850.12725283432730478No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCAGTACG4810.126203326415327No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4790.1256785724593381No Hit
ATCGAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC4780.1254161954813437No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4700.12331717965738814No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA4680.12279242570139925No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4680.12279242570139925No Hit
AGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAGACTA4550.11938152498747151No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC4520.11859439405348818No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4480.11754488614151039No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGAAC4470.11728250916351594No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAAT4450.11675775520752707No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4400.11544587031755485No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT4390.1151834933395604No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT4350.11413398542758264No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAATCTCCTCC4340.1138716084495882No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4270.1120349696036271No Hit
GTTTAATACCCTTATCCAAAGGCAAATATTTGGTAACATTTGGATAAAAC4250.11151021564763822No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4250.11151021564763822No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCA4230.11098546169164933No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4230.11098546169164933No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGCATACATTTAA4230.11098546169164933No Hit
CTTATATAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTTTCCA4230.11098546169164933No Hit
TCCTAGTACAAAGATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGT4230.11098546169164933No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4230.11098546169164933No Hit
ATAGAGTCCCAAGCGACCCCGAGAAGGGTCGTCCGCGGGATTCAGCGCCG4220.11072308471365488No Hit
GATTTGGTGGGCGTTCACGGTGGTTTCCATGCGACGTGCAGAGGTGAAGC4200.11019833075766601No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGTGC4200.11019833075766601No Hit
TAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCG4190.10993595377967157No Hit
AATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT4180.10967357680167712No Hit
GTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGACTGT4170.10941119982368268No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACCTAATGACTCTA4130.10836169191170492No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4110.107836937955716No Hit
GTATGGATCGGCAGAGGAGCCATAAAGGTTCCACGCATGCGCTGATGGCC4100.10757456097772156No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACCTAATGA4080.10704980702173268No Hit
GTACAAAGATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA4070.10678743004373824No Hit
CCTAATAGTCAGTTATGTCAACACTAATATGGGCCTAAAGTTTAGACAAC4040.1060002991097549No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCGGCGACGCGGCGA4010.10521316817577159No Hit
CTATTAGGTCTCTAGATGCTTGATCTTCCAAATTACCGCCCACCCAGGTA3980.10442603724178826No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3960.10390128328579937No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT3960.10390128328579937No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA3960.10390128328579937No Hit
ATATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGG3960.10390128328579937No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT3950.10363890630780492No Hit
GACGGGGAGGCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG3890.10206464443983827No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC3880.10180226746184383No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCTTTTGGGTTTT3820.10022800559387718No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)