FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381131
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC20190.52973911857078No Hit
ATATAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTTTCCATGC18390.4825112625317804No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATCAACTAGA16050.4211150496810808No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14720.3862189116078199No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13790.3618178526543367No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT11880.31170384985739813No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC11560.303307786561576No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT9700.25450566865460955No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9640.2529314067866429No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTAT8490.222758054317282No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATCAACTAGATGTTCTGGAT8290.21751051475739314No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8230.21593625288942644No Hit
AATATACATCGTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG8180.21462436799945422No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC8020.21042633635154318No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC7850.20596592772563765No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7660.20098076514374325No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7430.19494609464987103No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT7040.18471339250808777No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT6930.1818272457501489No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6930.1818272457501489No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6900.18104011481616558No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT6900.18104011481616558No Hit
GTATAAAGGGCCTCAAGATGCGGTACAGACTTGGCCCCCAATACCACATC6750.17710446014624892No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA6490.17028265871839343No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCATCAAACTCTGCAAG6480.17002028174039896No Hit
GTTATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCAAGC6110.16031233355460459No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCA6090.1597875795986157No Hit
ATATTAGTGTTGACATAACTGACTATTAGGTCTCTAGATGCTTGATCTTC5940.15585192492869906No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5890.15454004003872684No Hit
CACTAATATGGGCCTAAAGTTTAGACAACTCTTGTGGTTTCACATTTCTT5870.15401528608273796No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGATTTGGTGGGCGTTC5810.1524410242147713No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA5750.15086676234680466No Hit
ATGTATATTTGCGGGAGAGGACAACAGAGTTATCAGTCCCGATAATGTTT5740.1506043853688102No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTG5610.14719348465488244No Hit
CTCTTATATAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTTTC5520.14483209185293244No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5490.14404496091894914No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA5480.1437825839409547No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATAA5480.1437825839409547No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5440.14273307602897692No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5360.14063406020502137No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT5300.1390597983370547No Hit
CATTCTCGGGACTGATAACTCTGTTGTCCTCTCCCGCAAATATACATCGT5270.13827266740307137No Hit
GCCATAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC5260.13801029042507693No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT5210.13669840553510473No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5010.13145086597521588No Hit
CTATAAGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTC4950.12987660410724922No Hit
GTACCGCATCTTGAGGCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC4950.12987660410724922No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4950.12987660410724922No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCAGTACG4790.1256785724593381No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA4770.12515381850334925No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4700.12331717965738814No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGCATACATTTAA4680.12279242570139925No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG4660.12226767174541037No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4620.1212181638334326No Hit
GCCTAGCAGCCATGGGAACGATGTATATTTGCGGGAGAGGACAACAGAGT4600.12069340987744372No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAAT4580.12016865592145484No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA4570.1199062789434604No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4530.11885677103148262No Hit
ATCGAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACC4530.11885677103148262No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4430.11623300125153818No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT4390.1151834933395604No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA4390.1151834933395604No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACCTAATGACTCTA4370.11465873938357153No Hit
TACCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4370.11465873938357153No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4340.1138716084495882No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4310.11308447751560487No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC4250.11151021564763822No Hit
CCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT4220.11072308471365488No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGCCTTTTGGGTTTT4200.11019833075766601No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCA4190.10993595377967157No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC4160.10914882284568823No Hit
AGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAGACTA4160.10914882284568823No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT4150.10888644586769379No Hit
GTTTAATACCCTTATCCAAAGGCAAATATTTGGTAACATTTGGATAAAAC4110.107836937955716No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4090.10731218399972711No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGAAC4050.10626267608774935No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4040.1060002991097549No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCGGCGACGCGGCGA4040.1060002991097549No Hit
ATATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGG4030.10573792213176046No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACCTAATGA4010.10521316817577159No Hit
GTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGACTGT4010.10521316817577159No Hit
CTTTATGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTT4000.10495079119777714No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4000.10495079119777714No Hit
GTATGGATCGGCAGAGGAGCCATAAAGGTTCCACGCATGCGCTGATGGCC3970.10416366026379381No Hit
GTTTACGTCCCCTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGTCG3950.10363890630780492No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT3930.10311415235181604No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3910.10258939839582716No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAATCTAATCTCCTCC3870.10153989048384938No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3860.10127751350585494No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT3850.10101513652786051No Hit
CCCCAAAAGGCCTCCGTGTGGTGGGGTGAAACCCAGCCCGAATGCTCCAG3840.10075275954986607No Hit
CTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTTTTGCCTTCT3830.10049038257187162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)