FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410836
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA24410.5941543584301278No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13810.33614386275788877No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13410.32640761763818166No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT12690.3088823764227088No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT12570.3059615028867967No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12080.29403460261515546No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA10480.2550896221363269No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9850.23975503607278817No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT9540.23220944610501515No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTAGTAACATTTGGATAAAAC9530.23196603997702245No Hit
CCCTTATCCAATGGCAAATATTTAGTAACATTTGGATAAAACCTAGCAGG9320.22685451128917622No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT9150.22271660711330066No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT9020.21955232744939587No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8930.21736167229746173No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT8610.20957267620169603No Hit
ATGTAAGACCTTGGGCAAGAGGTGGTGGTTGTTCACGGTGGTCTCCATGC8380.20397433525786443No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACCCCCTCTTTTCCTAAT8170.1988628065700182No Hit
CTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAACACATAGCGCC8080.19667215141808408No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8050.19594193303410606No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7770.18912656145031106No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTAGTAACATT7730.18815293693834037No Hit
CACTAATATGGGCTTAAAGTTCAGACAACTTTTGTGGTTTCACATTTCTT7610.18523206340242823No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7530.1832848143784868No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7460.18158097148253804No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7290.1774430673066625No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTGTGT7200.1752524121547284No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAGGTGGTGGTTGTTC7090.17257494474680896No Hit
CAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAA6970.1696540712108968No Hit
CTATTTACACACTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAA6620.16113485673115308No Hit
CTCTTATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT6340.15431948514735808No Hit
GTGTATGATGTGTTCTTGTGGCAATGACCCATAACATCCAATGACATAAC6270.15261564225140933No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT6260.15237223612341663No Hit
CATCATACACAAAATCAAAGACTGTTTTAGAAAACTTCCTGTTAACAGGC6160.14993817484348987No Hit
TTCTAAAACAGTCTTTGATTTTGTGTATGATGTGTTCTTGTGGCAATGAC5950.1448266461556436No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT5900.14360961551568024No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACCCCCTCTTTTC5850.14239258487571682No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTG5630.1370376500598779No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5530.13460358877995113No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTT5520.13436018265195843No Hit
CTTATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT5400.13143930911604632No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5340.12997887234809025No Hit
CTCTAAGGCATCCCGATACAAAGCTGAGGCGGTATCTAGAAGATCTCGTA5270.12827502945214148No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACCCCCT5240.12754481106816346No Hit
CTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTTTTGCCTTCT5210.1268145926841854No Hit
CTCCGAAAGACACCAAATATTCTATAACTGTTTCTCTTCCAAAAGTGAGA5180.12608437430020739No Hit
GGATAAAACCTAGCAGGCATAATCAATTTCAATCTTCTTTTCTCATTAAC5100.12413712527626596No Hit
AATATGGGCTTAAAGTTCAGACAACTTTTGTGGTTTCACATTTCTTGTCT5090.12389371914827327No Hit
CCTTTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG5070.12340690689228793No Hit
GTTAATGAGAAAAGAAGATTGAAATTGATTATGCCTGCTAGGTTTTATCC5010.12194647012433185No Hit
GTTATGTCAACACTAATATGGGCTTAAAGTTCAGACAACTTTTGTGGTTT4990.12145965786834649No Hit
GGCAAATATTTAGTAACATTTGGATAAAACCTAGCAGGCATAATCAATTT4980.12121625174035382No Hit
GTGTAAATGTATATTAGGAAAAGAGGGGGTTTTCCAATGAGGATTAAAGA4950.12048603335637578No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4930.11999922110039042No Hit
CCCCTATCTTATCCACACTTCCGGAGACTACTGTTGTTAGACGACGAGGC4900.1192690027164124No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAA4860.11829537820444166No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4840.11780856594845633No Hit
TAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCG4810.11707834756447828No Hit
CATCTTTTGATAATGTCTTGGTGTAAATGTATATTAGGAAAAGAGGGGGT4760.11586131692451489No Hit
ATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA4750.1156179107965222No Hit
ATCCAAATGTTACTAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT4740.11537450466852953No Hit
ATCTTATCCACACTTCCGGAGACTACTGTTGTTAGACGACGAGGCAGGTC4700.11440088015655882No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGAAATTGATTA4680.11391406790057347No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4680.11391406790057347No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4670.1136706617725808No Hit
CTTTAATCCTCATTGGAAAACCCCCTCTTTTCCTAATATACATTTACACC4650.11318384951659542No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4640.11294044338860275No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4600.11196681887663204No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4590.11172341274863935No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4590.11172341274863935No Hit
ATCAAAGACTGTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAA4580.11148000662064668No Hit
GTTATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4540.11050638210867597No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4540.11050638210867597No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTAGTA4530.11026297598068328No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4520.1100195698526906No Hit
TCTAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCTTAAAGTT4470.10880253921272721No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4450.10831572695674187No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG4430.1078289147007565No Hit
AGATAGGGGCATTTGGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACT4420.10758550857276382No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4400.10709869631677849No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4400.10709869631677849No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4400.10709869631677849No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTTCAATCTTCTTTTCTCA4390.1068552901887858No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCA4370.10636847793280044No Hit
TTAATACCCTTATCCAATGGCAAATATTTAGTAACATTTGGATAAAACCT4340.1056382595488224No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGTGC4340.1056382595488224No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATT4330.10539485342082973No Hit
CAGTTAATGAGAAAAGAAGATTGAAATTGATTATGCCTGCTAGGTTTTAT4310.10490804116484437No Hit
GTTATGGGTCATTGCCACAAGAACACATCATACACAAAATCAAAGACTGT4300.10466463503685168No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG4290.10442122890885902No Hit
CTTCTAGATACCGCCTCAGCTTTGTATCGGGATGCCTTAGAGTCTCCTGA4270.10393441665287366No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG4250.10344760439688828No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4240.10320419826889562No Hit
TCCTAGTACAAAGACCTTTAACCTAGTCTCCTCCCCCAACTCCTCCCAGT4230.10296079214090295No Hit
GGGTTATTCTCTAAATTTTATGGGTTATGTCATTGGATGTTATGGGTCAT4230.10296079214090295No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAAT4220.10271738601291026No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4200.1022305737569249No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGGTGTAAATGTATATT4170.10150035537294687No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT4150.10101354311696151No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC4120.10028332473298349No Hit
ACCCATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCAT4120.10028332473298349No Hit
GTCTTTGATTTTGTGTATGATGTGTTCTTGTGGCAATGACCCATAACATC4120.10028332473298349No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTT4110.1000399186049908No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)