FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410836
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA22940.5583736576152042No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT13910.3385779240378156No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13710.333709801477962No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13430.326894429894167No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12570.3059615028867967No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT11380.2769961736556679No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT11170.2718846449678217No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT10880.264825867256034No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT10690.26020115082417317No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT10300.2507083118324587No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA10230.24900446893650996No Hit
CTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAACACATAGCGCC9790.2382945993048321No Hit
CCCTTATCCAATGGCAAATATTTAGTAACATTTGGATAAAACCTAGCAGG9530.23196603997702245No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTAGTAACATTTGGATAAAAC9450.23001879095308103No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT8760.21322376812158622No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8740.21273695586560087No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8640.21030289458567408No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACCCCCTCTTTTCCTAAT8610.20957267620169603No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7670.1866925001703843No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7650.18620568791439893No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA7540.18352822050647946No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTAGTAACATT7300.17768647343465518No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTGTGT7040.17135791410684556No Hit
CAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAA7010.17062769572286754No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACCCCCTCTTTTC6640.16162166898713842No Hit
GTGTATGATGTGTTCTTGTGGCAATGACCCATAACATCCAATGACATAAC6640.16162166898713842No Hit
CACTAATATGGGCTTAAAGTTCAGACAACTTTTGTGGTTTCACATTTCTT6450.15699695255527754No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGAAATTGATTA6280.152859048379402No Hit
CTCTTATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT6240.15188542386743129No Hit
ATGTAAGACCTTGGGCAAGAGGTGGTGGTTGTTCACGGTGGTCTCCATGC6120.14896455033151915No Hit
GTGTAAATGTATATTAGGAAAAGAGGGGGTTTTCCAATGAGGATTAAAGA6060.14750411356356308No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT6030.14677389517958503No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5980.14555686453962163No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTG5970.14531345841162896No Hit
CTATTTACACACTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAA5930.14433983389965827No Hit
CTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTTTTGCCTTCT5930.14433983389965827No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5910.1438530216436729No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT5900.14360961551568024No Hit
CTCCGAAAGACACCAAATATTCTATAACTGTTTCTCTTCCAAAAGTGAGA5890.14336620938768754No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAGGTGGTGGTTGTTC5820.1416623664917388No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5790.14093214810776078No Hit
CATCATACACAAAATCAAAGACTGTTTTAGAAAACTTCCTGTTAACAGGC5690.13849808682783396No Hit
TTCTAAAACAGTCTTTGATTTTGTGTATGATGTGTTCTTGTGGCAATGAC5510.13411677652396578No Hit
CTCTAAGGCATCCCGATACAAAGCTGAGGCGGTATCTAGAAGATCTCGTA5460.13289974588400236No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG5340.12997887234809025No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5330.12973546622009757No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5330.12973546622009757No Hit
CTTATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT5300.12900524783611952No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5240.12754481106816346No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5170.1258409681722147No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5170.1258409681722147No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5070.12340690689228793No Hit
CCCCTATCTTATCCACACTTCCGGAGACTACTGTTGTTAGACGACGAGGC5060.12316350076429525No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5050.12292009463630256No Hit
GTTATGTCAACACTAATATGGGCTTAAAGTTCAGACAACTTTTGTGGTTT4970.12097284561236113No Hit
CATCTTTTGATAATGTCTTGGTGTAAATGTATATTAGGAAAAGAGGGGGT4940.12024262722838311No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAA4910.11951240884440506No Hit
GGATAAAACCTAGCAGGCATAATCAATTTCAATCTTCTTTTCTCATTAAC4890.11902559658841971No Hit
CCTTTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG4880.11878219046042704No Hit
ATATAAGATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA4870.11853878433243435No Hit
TAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCG4860.11829537820444166No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4810.11707834756447828No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCA4810.11707834756447828No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTT4800.1168349414364856No Hit
GGCAAATATTTAGTAACATTTGGATAAAACCTAGCAGGCATAATCAATTT4790.11659153530849294No Hit
GTATGCATGTATTCAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACA4750.1156179107965222No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTT4750.1156179107965222No Hit
GTTAATGAGAAAAGAAGATTGAAATTGATTATGCCTGCTAGGTTTTATCC4720.11488769241254418No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4680.11391406790057347No Hit
GTCTTTGATTTTGTGTATGATGTGTTCTTGTGGCAATGACCCATAACATC4610.11221022500462471No Hit
ATCAAAGACTGTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAA4600.11196681887663204No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4590.11172341274863935No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTAGTAACATTTGGATAAAA4590.11172341274863935No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTAGTA4540.11050638210867597No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4490.10928935146871258No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACCCCCT4470.10880253921272721No Hit
AGATAGGGGCATTTGGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACT4470.10880253921272721No Hit
CTTCTAGATACCGCCTCAGCTTTGTATCGGGATGCCTTAGAGTCTCCTGA4460.10855913308473455No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4460.10855913308473455No Hit
CAGTTAATGAGAAAAGAAGATTGAAATTGATTATGCCTGCTAGGTTTTAT4420.10758550857276382No Hit
CTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGGA4420.10758550857276382No Hit
TCCTAGTACAAAGACCTTTAACCTAGTCTCCTCCCCCAACTCCTCCCAGT4390.1068552901887858No Hit
GTTATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4390.1068552901887858No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAGTCTCCTCC4340.1056382595488224No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATT4300.10466463503685168No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTCTCATCTGCCGGCCCGTGTG4300.10466463503685168No Hit
CCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTTTTGCCTT4300.10466463503685168No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC4290.10442122890885902No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAAT4270.10393441665287366No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTTCAATCTTCTTTTCTCA4240.10320419826889562No Hit
CTTTAATCCTCATTGGAAAACCCCCTCTTTTCCTAATATACATTTACACC4220.10271738601291026No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4200.1022305737569249No Hit
ATCTTATCCACACTTCCGGAGACTACTGTTGTTAGACGACGAGGCAGGTC4200.1022305737569249No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG4170.10150035537294687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)