FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605301
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA56150.9276376546544611No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37540.6201873117672034No Hit
AGTCTGTACAGCATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT21930.36229908756139506No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT17580.29043401547329345No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTT17440.2881211166014925No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT16780.2772174504915736No Hit
GTGTAAAAGGGGCGGCAAAACCCAAAAGACCCACAATACGTTGACAGACT16460.27193082449888567No Hit
CTCTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA16150.2668094055684692No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC15820.26135757251350983No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15110.24962787109223347No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT15080.24913224990541896No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG13680.22600326118740927No Hit
CATTTACACCAAGACATTATTAAAAAATGTGAACAATTTGTAGGCCCACT13310.21989059988336385No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT12920.21344752445477538No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC12620.20849131258663048No Hit
ATATTGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAATCC12220.20188303009577055No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG12120.20023095947305555No Hit
ATAAACTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTA12020.19857888885034058No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT12000.19824847472579757No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA11670.19279664167083815No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC11550.19081415692358017No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC11470.1894925004254082No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG11300.18668398036679273No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT11190.18486670268180624No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT11130.18387546030817725No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT10920.1804061120004758No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC10820.1787540413777608No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT10750.17759759194186034No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT10640.17578031425687385No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT10470.1729717941982584No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC10310.17032848120191443No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9890.1633897845865115No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9810.16206812808833954No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTT9710.16041605746562454No Hit
CATTTAAATCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT9520.15727712328246607No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT9430.1557902597220226No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA9370.1547990173483936No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT9150.15116446197842065No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC9070.14984280548024867No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA8970.1481907348575337No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG8750.14455617948756072No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA8730.14422576536301773No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8690.14356493711393173No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8680.14339973005166026No Hit
CACCAAGACATTATTAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT8660.14306931592711725No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT8620.14240848767803127No Hit
GTACAGCATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8520.14075641705531627No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8340.13778268993442933No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC8250.13629582637398582No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAATCCCTTTTTACC8210.13563499812489985No Hit
CTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT8190.13530458400035683No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG8160.13480896281354235No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC8030.13266127100401287No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT7980.1318352356926554No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA7700.12720943794905343No Hit
ATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAATCC7630.12605298851315294No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG7430.12274884726772299No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA7420.1225836402054515No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC7300.12060115545819351No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATGATTAATCT7210.11911429189775005No Hit
GGATAACCACATTGTGTAAAAGGGGCGGCAAAACCCAAAAGACCCACAAT7190.11878387777320705No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC7120.11762742833730656No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG7080.11696660008822057No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGATTTAAATGTATACCC7060.11663618596367757No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA7050.11647097890140608No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7040.11630577183913458No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC7030.11614056477686308No Hit
ACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTTAGGCAA6860.11333204471824761No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA6820.11267121646916162No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGATTTAAATGTATACCCAAAGA6820.11267121646916162No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6820.11267121646916162No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATAC6810.11250600940689014No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG6780.11201038822007564No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT6660.11002790347281766No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA6590.10887145403691717No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA6520.10771500460101668No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6470.1068889692896592No Hit
CTCCGAAAGACACCAAATACTCTATAACCGTTTCTCTTCCAAAAGTGAGA6410.10589772691603021No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT6350.10490648454240122No Hit
GTCTTTGGGTATACATTTAAATCCTAACAAAACAAAAAGATGGGGTTACT6340.10474127748012974No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG6320.10441086335558672No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGATTTAAATGTATAC6300.10408044923104372No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT6260.10341962098195773No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATTCATTTACACC6260.10341962098195773No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG6250.10325441391968625No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6250.10325441391968625No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG6210.10259358567060026No Hit
GCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC6200.10242837860832874No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT6170.10193275742151424No Hit
ACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT6170.10193275742151424No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT6160.10176755035924276No Hit
TAACCACATTGTGTAAAAGGGGCGGCAAAACCCAAAAGACCCACAATACG6150.10160234329697126No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6130.10127192917242826No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC6120.10110672211015677No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6100.10077630798561377No Hit
GGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAA6070.10028068679879927No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC6060.10011547973652778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)