FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605301
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA50150.8285134172915624No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37470.6190308623313029No Hit
AGTCTGTACAGCATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT21150.3494129367042182No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT17730.29291212140736594No Hit
CTCTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA16890.27903472817656005No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTT16210.26780064794209824No Hit
GTGTAAAAGGGGCGGCAAAACCCAAAAGACCCACAATACGTTGACAGACT15970.2638356784475823No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT15240.2517755629017629No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT14890.2459933157222605No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG14670.24235876035228754No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT14350.2370721343595996No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC14290.2360808919859706No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC13970.23079426599328265No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC13730.22682929649876674No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13200.21807332219837733No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG12480.2061784137148295No Hit
CATTTACACCAAGACATTATTAAAAAATGTGAACAATTTGTAGGCCCACT11980.19791806060125458No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA11690.19312705579538114No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG11520.19031853573676566No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT11480.18965770748767968No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT11180.18470149561953475No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT11000.18172776849864777No Hit
ATATTGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAATCC10890.17991049081366128No Hit
ATAAACTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTA10700.17677155663050284No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT10660.17611072838141684No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT9750.16107688571471052No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTT9660.15959002215426704No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC9620.15892919390518107No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT9380.1549642244106651No Hit
GTACAGCATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9140.15099925491614916No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9050.14951239135570568No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT9000.14868635604434818No Hit
CTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT9000.14868635604434818No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC8800.14538221479891825No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8670.14323452298938877No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8640.14273890180257426No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT8620.14240848767803127No Hit
GGATAACCACATTGTGTAAAAGGGGCGGCAAAACCCAAAAGACCCACAAT8540.1410868311798593No Hit
CATTTAAATCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8410.1389391393703298No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG8390.1386087252457868No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA8220.13580020518717134No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC8170.13497416987581387No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA8170.13497416987581387No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC8090.13365251337764186No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC8060.13315689219082738No Hit
CACCAAGACATTATTAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT8060.13315689219082738No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT7960.13150482156811238No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7950.1313396145058409No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC7940.1311744074435694No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA7920.1308439933190264No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAATCCCTTTTTACC7890.1303483721322119No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG7880.1301831650699404No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7790.12869630150949693No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA7740.12787026619813943No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7660.12654860969996745No Hit
CTCCGAAAGACACCAAATACTCTATAACCGTTTCTCTTCCAAAAGTGAGA7600.12555736732633846No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7580.12522695320179547No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATGATTAATCT7550.12473133201498098No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA7390.122088019018637No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT7210.11911429189775005No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7170.11845346364866406No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA7170.11845346364866406No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC7120.11762742833730656No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG7030.11614056477686308No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6960.11498411534096259No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6950.1148189082786911No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6900.1139928729673336No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTATGGA6850.11316683765597611No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGATTTAAATGTATAC6850.11316683765597611No Hit
GGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAA6840.11300163059370463No Hit
ATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAATCC6800.11234080234461861No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGATTTAAATGTATACCC6750.11151476703326114No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6680.11035831759736066No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC6640.10969748934827467No Hit
ACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT6590.10887145403691717No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA6590.10887145403691717No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGATTTAAATGTATACCCAAAGA6550.10821062578783118No Hit
GCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC6500.10738459047647368No Hit
ACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTTAGGCAA6490.10721938341420219No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6490.10721938341420219No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA6440.1063933481028447No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC6430.1062281410405732No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT6350.10490648454240122No Hit
GTCTTTGGGTATACATTTAAATCCTAACAAAACAAAAAGATGGGGTTACT6350.10490648454240122No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC6340.10474127748012974No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG6310.10424565629331524No Hit
ATATTTGCCATTGGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG6260.10341962098195773No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAA6200.10242837860832874No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA6180.10209796448378576No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG6140.10143713623469977No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT6120.10110672211015677No Hit
CTATTGTGGTTTCACATTTCTTGTCTCACTTTTGGAAGAGAAACGGTTAT6100.10077630798561377No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC6070.10028068679879927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)