FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501615
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA41350.8243373902295585No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT25250.5033741016516652No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT20390.40648704683871095No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16490.3287381756925132No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15720.3133877575431357No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG15110.3012270366715509No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC14540.2898637401194143No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT14340.2858766185221734No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC14020.27949722396658794No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGT13960.27830108748741567No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT13600.27112426861238204No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTCTTGTGGTTTCACATTTCTT13410.2673365030950031No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT13260.26434616189707244No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG13230.2637480936574863No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA13150.26215324501858994No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT12760.2543783579039702No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT12490.24899574374769493No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTT12120.24161956879279928No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCACTCAGGCA12110.2414202127129372No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA11260.22447494592466333No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT11250.22427558984480128No Hit
GTTTGTAGGCCCACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTA11150.22228202904618083No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC10960.21849426352880197No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC10760.21450714193156106No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC10690.21311164937252675No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT10590.21111808857390627No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA10460.2085264595356997No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC10350.2063335426572172No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10210.20354255753914854No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC9970.19875801162245946No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9890.1971631629835631No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT9690.19317604138632216No Hit
CTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA9540.1901857001883915No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA9150.18241081307377172No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT8970.1788224036362549No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT8910.17762626715708263No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT8680.1730410773202556No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8660.17264236516053147No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT8280.16506683412577375No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCATCAAACTCTGCAAG8180.1630732733271533No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT8090.1612790686083949No Hit
GTGTATGATGTGTTCTTGTGGCAATGATCCATAACATCCAATGACATAAC7890.15729194701115395No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA7840.15629516661184376No Hit
CTCCGAAAGACACCAAATATTCTATAACTGTTTCTCTTCCAAAAGTGAGA7740.15430160581322327No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7720.15390289365349918No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA7700.1535041814937751No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7660.15270675717432694No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTG7630.15210868893474078No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA7490.14931770381667214No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTGTGT7460.148719635577086No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG7440.14832092341736192No Hit
GTCATAGGCCATCAGCGCATGCGTGGAACCTTTATGGCTCCTCTGCCGAT7430.14812156733749987No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG7300.14552993829929328No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT7290.14533058221943124No Hit
GCCTATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC7190.14333702142081078No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG7070.14094474846246624No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7070.14094474846246624No Hit
GTTATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6960.13875183158398371No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGGTGTAAATGTATATT6890.13735633902494943No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG6870.1369576268652253No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6820.1359608464659151No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTTTCA6810.13576149038605306No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA6740.13436599782701875No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6720.13396728566729466No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATAA6710.13376792958743258No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6650.13257179310826034No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC6600.13157501270895008No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6560.13077758838950193No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT6550.13057823230963989No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTT6540.1303788762297778No Hit
GGCATAATCAATTGCAATCTTCTTTTTTCATTAACTGTGAGTGGGCCTAC6530.13017952014991577No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6470.1289833836707435No Hit
GTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA6400.12758789111170918No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6380.1271891789519851No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT6320.12599304247281282No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG6310.12579368639295077No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6210.1238001255943303No Hit
GTCTCCACCTTTGCGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCA6190.12340141343460621No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG6170.12300270127488214No Hit
TTGTAGGCCCACTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTATG6150.12260398911515805No Hit
CATCATACACAAAATCAAAGACTGTTTTAGAAAACTTCCTGTTAACAGGC6100.1216072087158478No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAATTAGAGGACAAACGGGCAAC6090.12140785263598576No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC6030.12021171615681349No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTG6000.11961364791722734No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGGCCCACAAT5920.11801879927833099No Hit
CTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTTTTGCCTTCT5900.11762008711860689No Hit
GTATGGATCGGCAGAGGAGCCATAAAGGTTCCACGCATGCGCTGATGGCC5880.11722137495888281No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC5840.11642395063943463No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5800.11562652631998645No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5770.11502845808040031No Hit
GGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCGATTGGTGGAGGC5760.11482910200053827No Hit
CTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCT5760.11482910200053827No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5760.11482910200053827No Hit
GCCTGAGGATGAGTGTTTCGCAAAGGTGGAGACAGCGGGGTAGGCTGCCT5730.11423103376095212No Hit
GCCATAAAGGTTCCACGCATGCGCTGATGGCCTATGACCAAGCCCCAGCC5720.11403167768109007No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTCCGACCGACCACGGGGCGCACCT5700.113632965521366No Hit
GTCTTTGATTTTGTGTATGATGTGTTCTTGTGGCAATGATCCATAACATC5680.1132342533616419No Hit
TTCTAAAACAGTCTTTGATTTTGTGTATGATGTGTTCTTGTGGCAATGAT5640.11243682904219371No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA5630.11223747296233166No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5550.1106426243234353No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5480.10924713176440098No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5470.10904777568453894No Hit
CCTCCAGCTTATAGACCACCAAATATTCTATAACTGTTTCTCTTCCAAAA5460.1088484196046769No Hit
CTCACAGTTAATGAAAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT5450.10864906352481485No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAAATCTTGCCC5450.10864906352481485No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC5450.10864906352481485No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5450.10864906352481485No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCTTAT5430.10825035136509074No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5380.10725357096578053No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATT5380.10725357096578053No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5350.10665550272619438No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5340.10645614664633234No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5310.10585807840674621No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG5290.10545936624702212No Hit
GTTATGTCAACACTAATATGGGCCTAAAGTTCAGACAACTCTTGTGGTTT5270.10506065408729802No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5250.10466194192757393No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5250.10466194192757393No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5230.10426322976784985No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTC5230.10426322976784985No Hit
CAGTTAATGAAAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT5190.10346580544840166No Hit
GTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACA5170.10306709328867757No Hit
ATGTATATTTGCGGGAGAGGACAACAGAGTTATCAGTCCCGAGAATGTTT5150.10266838112895348No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTG5140.10246902504909144No Hit
GTTATGGATCATTGCCACAAGAACACATCATACACAAAATCAAAGACTGT5140.10246902504909144No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5140.10246902504909144No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5130.1022696689692294No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5110.1018709568095053No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA5040.10047546425047099No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCTTGTTCCGA5030.10027610817060895No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG5030.10027610817060895No Hit
GGCCAGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGC5030.10027610817060895No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGTGC5030.10027610817060895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)