FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487748
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33730.6915456342209502No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA21830.4475671863339265No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATGATTAATCT13230.27124662735674976No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10900.22347605730828213No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT10830.22204088996777022No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10210.2093294078089505No Hit
CACTTATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9510.19497773440383148No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9340.1914923280054454No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8590.17611553507138933No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGGCCACT8550.17529543944823966No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8100.166069363687806No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA7620.1562282162100101No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA7520.15417797715213594No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC7460.15294783371741144No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCGGACTTCTTTCCTTCAGTACG7430.1523327620000492No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7380.1513076424711121No Hit
GTCCAAGAGTCCTCTTATGTAAGACAGAGAGTCCAAGAGTCCTCTTATGT7250.14864233169587573No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7240.14843730779008832No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7220.1480272599785135No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAAACACCGTG7210.14782223607272607No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7040.14433682967434003No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC6880.1410564471817414No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG6880.1410564471817414No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT6860.14064639937016657No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6610.13552080172548117No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6270.12854998892870909No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA6180.12670477377662237No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6180.12670477377662237No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTTTGT6170.12649974987083495No Hit
GCTGTAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGAC6170.12649974987083495No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6120.12547463034189787No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6080.12465453471874821No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC5930.12157917613193699No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA5880.1205540566029999No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5810.119118889262488No Hit
ATTGATGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGA5800.11891386535670058No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5700.11686362629882645No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5660.11604353067567678No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5620.11522343505252712No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCGA5510.11296817208886556No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5480.11235310037150331No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5460.11194305255992848No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5450.11173802865414106No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC5430.11132798084256623No Hit
CATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5420.11112295693677882No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTG5400.11071290912520401No Hit
CTCTTTACATTTCATGGGGTATGTCATTGGAAGTTATGGGTCATTACCAC5390.1105078852194166No Hit
GACATACCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG5370.11009783740784176No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA5260.10784257444418019No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5250.10763755053839277No Hit
CGGATAAGGGGACGAGAGAGTCCCAAGCGGCCCCGAGAAGGGTCGTCCGC5200.10661243100945571No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA5170.10599735929209346No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5140.10538228757473121No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5130.1051772636689438No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5120.10497223976315638No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCCGAAGGCAAAAACGAGA5080.10415214414000672No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5060.10374209632843188No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5050.10353707242264447No Hit
GTAATGACCCATAACTTCCAATGACATACCCCATGAAATGTAAAGAGTAA5040.10333204851685705No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5040.10333204851685705No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5010.1027169767994948No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT4980.10210190508213259No Hit
CCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCTCTCTTTAC4970.10189688117634517No Hit
GGGTATGTCATTGGAAGTTATGGGTCATTACCACAAGATCACATCAGACA4950.10148683336477034No Hit
ACTCTTTACATTTCATGGGGTATGTCATTGGAAGTTATGGGTCATTACCA4950.10148683336477034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)