FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545209
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA54701.003284978787951No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50060.9181800006969804No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT22560.413786272787133No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC15290.2804429127178752No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT15020.27549068338930577No Hit
GTTTAATACCCTTGTCCAATGGCAAATATTTGGTAACATTTGGATAAAAC14980.2747570197850732No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT14750.27053845406073634No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT14000.25678226148137684No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT13930.25549835017397No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT13740.2520134480538656No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC13340.2446768120115405No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT13200.2421089893967268No Hit
GGATAATAAGGTTTAATACCCTTGTCCAATGGCAAATATTTGGTAACATT12230.22431764699408852No Hit
CTAACAATCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT12080.2215664084782166No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA11250.20634288869039213No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTAACAAT11170.20487556148192712No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA11080.20322481837240397No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10480.19221986430891638No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10440.1914862007046839No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10340.18965204169410263No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT10310.18910179399092825No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGACAAGGGTATTAAACCTTAT10210.187267634980347No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10070.1846998123655332No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG9580.17571243321368502No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT9440.17314461059887126No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT9190.16855921307241808No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8890.1630567360406743No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA8870.16268990423855806No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT8870.16268990423855806No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCAATGGAAAACACCCTCTTTTC8850.1623230724364418No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8630.15828792261316302No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT8480.15553668409729113No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATAC8440.15480302049305864No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTATGGA8100.14856687985708233No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT8010.14691613674755918No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7940.1456322254401523No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG7920.14526539363803606No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG7920.14526539363803606No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7720.14159707561687354No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGACAAGGGTATTAAAC7580.13902925300205976No Hit
GTTCTGGATAATAAGGTTTAATACCCTTGTCCAATGGCAAATATTTGGTA7560.13866242119994351No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT7400.1357277667830135No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTC7270.13334336006925784No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7190.13187603286079283No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7080.12985845794915343No Hit
GGACAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7030.1289413784438628No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGAACAACAGAGT6790.12453939681846779No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAAT6520.11958716748989838No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC6500.1192203356877821No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCAATGGAAAACACCCT6480.11885350388566587No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6450.11830325618249148No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6370.11683592897402646No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6340.1162856812708521No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC6270.1150017699634452No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCATTGAGGGTTAAAGA6200.11371785865603834No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6150.11280077915074768No Hit
GGCATAATCAATTGCAGTCTTCTTTTCTCATTGATTGTTAGTGGGCCTAC6120.11225053144757333No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT6080.1115168678433408No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA6070.1113334519422827No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA6050.11096662014016642No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC6010.11023295653593392No Hit
CTAATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTA6000.11004954063487579No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5960.10931587703064329No Hit
ATATTTGCCATTGGACAAGGGTATTAAACCTTATTATCCAGAACATCTAG5940.10894904522852705No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA5910.10839879752535266No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC5830.10693147031688764No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5830.10693147031688764No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG5750.10546414310842266No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG5730.10509731130630638No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA5710.10473047950419015No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT5710.10473047950419015No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT5710.10473047950419015No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5710.10473047950419015No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5660.10381339999889952No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC5650.10362998409784137No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT5630.10326315229572514No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT5620.10307973639466701No Hit
ATTTGTAGGCCCACTAACAATCAATGAGAAAAGAAGACTGCAATTGATTA5620.10307973639466701No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5620.10307973639466701No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT5570.10216265688937638No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTAAGA5560.10197924098831823No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA5560.10197924098831823No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA5530.10142899328514388No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC5530.10142899328514388No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC5510.1010621614830276No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA5500.10087874558196948No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC5490.10069532968091137No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT5490.10069532968091137No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT5490.10069532968091137No Hit
GTCTTTAACCCTCAATGGAAAACACCCTCTTTTCCTAATATACATTTACA5470.1003284978787951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)