FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545209
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA50620.9284512911562355No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49950.916162425785341No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT20400.3741684381585777No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT16350.29988499823003656No Hit
GTTTAATACCCTTGTCCAATGGCAAATATTTGGTAACATTTGGATAAAAC16240.29786742331839716No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC15270.2800760809157589No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT14680.26925454275332944No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT14640.26852087914909695No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT13970.2562320137782025No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT13510.24779488232952868No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT13390.24559389151683117No Hit
GGATAATAAGGTTTAATACCCTTGTCCAATGGCAAATATTTGGTAACATT13320.2443099802094243No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC13220.24247582119884303No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12020.22046591307186783No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11210.2056092250861596No Hit
CTAACAATCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT11030.20230773886711334No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTAAGACAAGAAATGTGAAACCACA10600.19442085512161392No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA10590.19423743922055578No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10450.19166961660574203No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGACAAGGGTATTAAACCTTAT10360.19001887349621888No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT9880.18121491024542882No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9870.18103149434437069No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTAACAAT9830.1802978307401382No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9600.1760792650158013No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG9060.16617480635866247No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT8810.1615894088322093No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG8570.15718742720681428No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT8560.15700401130575614No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTATGGA8530.15645376360258176No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT8520.15627034770152362No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG8430.1546196045920005No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCAATGGAAAACACCCTCTTTTC8240.1511347024718961No Hit
GTTCTGGATAATAAGGTTTAATACCCTTGTCCAATGGCAAATATTTGGTA8200.1504010388676636No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTGGACAAGGGTATTAAAC8170.1498507911644892No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8090.1483834639560242No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG7860.1441648982316873No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA7830.1436146505285129No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATAC7710.1414136597158154No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT7630.1399463325073504No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7260.1331599441681997No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7200.13205944876185097No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7190.13187603286079283No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT6920.12692380353222343No Hit
GGACAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6880.1261901399279909No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA6630.12160474240153775No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC6620.12142132650047963No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCAATGGAAAACACCCT6560.12032083109413089No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGAACAACAGAGT6520.11958716748989838No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6360.11665251307296834No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC6330.11610226536979396No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6310.11573543356767771No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCATTGAGGGTTAAAGA6280.11518518586450333No Hit
CATTTGGATAAAACCTAGCAGGCATAATCAATTGCAGTCTTCTTTTCTCA6240.11445152226027082No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC6220.11408469045815457No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6220.11408469045815457No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6180.11335102685392207No Hit
GGCATAATCAATTGCAGTCTTCTTTTCTCATTGATTGTTAGTGGGCCTAC6140.11261736324968956No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC6000.11004954063487579No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTC5950.10913246112958516No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTAAGA5880.10784854982217827No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5780.10601439081159701No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5750.10546414310842266No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC5740.1052807272073645No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5740.1052807272073645No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT5720.10491389540524826No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA5650.10362998409784137No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAAT5650.10362998409784137No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGC5580.1023460727904345No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT5570.10216265688937638No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5560.10197924098831823No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC5550.10179582508726011No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5550.10179582508726011No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5520.10124557738408572No Hit
ACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5500.10087874558196948No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA5500.10087874558196948No Hit
ATTTGTAGGCCCACTAACAATCAATGAGAAAAGAAGACTGCAATTGATTA5470.1003284978787951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)