FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535312
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32970.6159025017186239No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT19590.36595480766356814No Hit
GTGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT17550.3278461906327525No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA14850.27740831515079056No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT12990.2426622231521057No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTCTCCATGC12790.2389260842275159No Hit
CAATAGGCCTGTTAACGGGAAGTTTTCTAAAACAGTCTTTGATTTTTTGT12140.22678363272259916No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA12040.22491556326030426No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTGATGCCCTTGTATGCATGTAT11380.21258630480915802No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA10990.20530083390620799No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT10880.2032459574976836No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC10650.19894939773440534No Hit
GACTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTG10160.1897958573691604No Hit
GGATAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAAT10100.1886750156917835No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9930.18549929760588219No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT9740.1819499656275219No Hit
CTTTCCAATCAATAGGCCTGTTAACGGGAAGTTTTCTAAAACAGTCTTTG9690.18101593089637447No Hit
TTCTAAAACAGTCTTTGATTTTTTGTATGATGTGTTCTTGTGGCAATGAC9600.17933466838030904No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9510.17765340586424366No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACT9080.16962070717637565No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC8890.16607137519801535No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTTTTGTGGTTTCACATTTCTT8790.16420330573572048No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA8670.16196162238096662No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT7940.14832471530621394No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7630.1425336999730998No Hit
TAACCACATTGTGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAATTCG7580.14159966524195236No Hit
GTTATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGACTGT7500.14010520967211645No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7340.13711629853244464No Hit
AATATGGGCCTAAAGTTCAGACAACTTTTGTGGTTTCACATTTCTTGTCT7280.1359954568550677No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCTGG7080.13225931793047793No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7060.13188570403801894No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG7000.13076486236064203No Hit
CTCCGAAAGACACCAAATATTCTATAACTGTTTCTCTTCCAAAAGTGAGA6940.12964402068326508No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6930.1294572137370356No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6920.12927040679080612No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6710.12534746091998686No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6650.12422661924260993No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6580.12291897061900349No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG6570.12273216367277402No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6540.12217174283408554No Hit
TGGGCATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTA6410.1197432525331022No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCTTTTGGGTTTT6410.1197432525331022No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6350.11862241085572527No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT6290.11750156917834834No Hit
GGGTTATTCTCTAAATTTTATGGGTTATGTCATTGGATGTTATGGGTCAT6230.11638072750097139No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6170.11525988582359446No Hit
ACCCATAACATCCAATGACATAACCCATAAAATTTAGAGAATAACCCCAT6170.11525988582359446No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC6120.11432585109244701No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6100.11395223719998804No Hit
GAATACATGCATACAAGGGCATCAAAGCAGGATAACCACATTGTGTAAAA6090.11376543025375856No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG6060.11320500941507008No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6020.11245778163015213No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5840.10909525659802136No Hit
GTTATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5840.10909525659802136No Hit
CTCTTTACGCGGCCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5820.10872164270556237No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC5820.10872164270556237No Hit
TTCTTCTACTGTGCCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC5800.10834802881310339No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5760.10760080102818544No Hit
CATGCATACAAGGGCATCAAAGCAGGATAACCACATTGTGTAAAAGGTGC5760.10760080102818544No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5710.10666676629703799No Hit
CCAAATATTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGA5660.10573273156589055No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAA5560.10386466210359567No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAA5560.10386466210359567No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG5530.10330424126490718No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTG5510.10293062737244821No Hit
AATTTGGGCCTAAAGTTCAGACAACTTTTGTGGTTTCACATTTCTTGTCT5440.1016229787488418No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC5440.1016229787488418No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5420.10124936485638282No Hit
GTATGGATCGGCAGAGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC5420.10124936485638282No Hit
ATGTATATTTGCGGGAGAGGACAACAGAGTTATCAGTCCCGAGAATGTTT5390.10068894401769435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)